The fitness effects of codon composition of the horizontally transferred antibiotic resistance genes intensify at sub-lethal antibiotic levels

M Shaferman, M Gencel, N Alon… - Molecular Biology …, 2023 - academic.oup.com
The rampant variability in codon bias existing between bacterial genomes is expected to
interfere with horizontal gene transfer (HGT), a phenomenon that drives bacterial adaptation. …

[HTML][HTML] Simplified methodology for a modular and genetically expanded protein synthesis in cell-free systems

Y Chemla, E Ozer, M Shaferman, B Zaad… - Synthetic and systems …, 2019 - Elsevier
Genetic code expansion, which enables the site-specific incorporation of unnatural amino
acids into proteins, has emerged as a new and powerful tool for protein engineering. Currently…

Genetic Code Expansion in the Service of Bioelectrochemistry

…, Y Cohen, I Moshel, M Shaferman… - Electrochemical …, 2023 - iopscience.iop.org
Genetic code expansion is a method that enables the inclusion of unnatural amino acids (UAAs)
into proteins by expanding the genetic code beyond the 20 canonical amino acids. This …

Expanding the genetic code of a photoautotrophic organism

Y Chemla, M Friedman, M Heltberg, A Bakhrat… - Biochemistry, 2017 - ACS Publications
The photoautotrophic freshwater cyanobacterium Synechococcus elongatus is widely used
as a chassis for biotechnological applications as well as a photosynthetic bacterial model. In …

[HTML][HTML] Continuous-time least-squares forgetting algorithms for indirect adaptive control

V Shaferman, M Schwegel, T Glück, A Kugi - European Journal of Control, 2021 - Elsevier
Many variants of least-squares forgetting algorithms can be found in the literature. They all
have advantages and disadvantages with respect to adaptation rate, robustness against noise…

[HTML][HTML] Genetic Code Expansion of Vibrio natriegens

E Ozer, L Alfonta - Frontiers in bioengineering and biotechnology, 2021 - frontiersin.org
… Ashok Kumar Bhagat and Michael Shaferman are acknowledged for technical assistance.
We would like to thank the Ilse Katz Institute for Nanoscale Science and Technology Shared …

In vitro suppression of two different stop codons

E Ozer, Y Chemla, O Schlesinger… - Biotechnology and …, 2017 - Wiley Online Library
Michael Shaferman is acknowledged for technical support. We would also like to
acknowledge Prof. Vincent Noiraeux for generously giving us the pBEST plasmid and for useful …

Genetically expanded reactive-oxygen-tolerant alcohol dehydrogenase II

AK Bhagat, H Buium, G Shmul, L Alfonta - ACS Catalysis, 2020 - ACS Publications
Herein, we report the site-specific incorporation of l-3,4-dihydroxyphenylalanine as a
promising method to engineer an oxygen-tolerant alcohol dehydrogenase II. The engineered …

Adaptive Two-Degrees-of-Freedom Current Control for Solenoids: Theoretical Investigation and Practical Application

M Schwegel, T Glück, V Shaferman… - … on Control Systems …, 2022 - ieeexplore.ieee.org
In this article, an adaptive two-degrees-of-freedom current control algorithm for solenoids is
presented comprising an adaptive pole placement controller in combination with a …

Toxic metals and essential elements contents in commercially available fruit juices and other non-alcoholic beverages from the United States

…, M Johnson, K Cowden, D Shaferman… - Journal of Food …, 2023 - Elsevier
Elevated levels of toxic metals and essential elements in commonly consumed soft beverages
are of public and regulatory agencies’ concern, and thus frequent monitoring of these …