Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis thaliana,
zebrafish and rats, efficient genome engineering in human embryonic stem and induced …

Nucleases that cleave unique genomic sequences in living cells can be used for targeted
gene editing and mutagenesis. Here we develop a strategy for generating such reagents …

Mendelian randomization (MR) is a term that applies to the use of genetic variation to
address causal questions about how modifiable exposures influence different outcomes. The …

Permanent modification of the human genome in vivo is impractical owing to the low frequency
of homologous recombination in human cells, a fact that hampers biomedical research …

Background CCR5 is the major coreceptor for human immunodeficiency virus (HIV). We
investigated whether site-specific modification of the gene (“gene editing”) — in this case, the …

Mendelian randomisation uses genetic variation as a natural experiment to investigate the
causal relations between potentially modifiable risk factors and health outcomes in …

Genome editing driven by zinc-finger nucleases (ZFNs) yields high gene-modification
efficiencies (>10%) by introducing a recombinogenic double-strand break into the targeted gene. …

We present a draft sequence of the genome of Aedes aegypti, the primary vector for yellow
fever and dengue fever, which at ∼1376 million base pairs is about 5 times the size of the …

Homozygosity for the naturally occurring Δ32 deletion in the HIV co-receptor CCR5 confers
resistance to HIV-1 infection. We generated an HIV-resistant genotype de novo using …

The incorporation of histone H3 variants has been implicated in the epigenetic memory of
cellular state. Using genome editing with zinc-finger nucleases to tag endogenous H3.3, we …