In many species, introduction of double-stranded RNA (dsRNA) induces potent and specific
gene silencing, a phenomenon called RNA interference or RNAi. The apparently …

The ‘sterol-sensing domain' (SSD) is conserved across phyla and is present in several
membrane proteins, such as Patched (a Hedgehog receptor) and NPC-1 (the protein defective in …

Epithelial cells play a central role in many embryonic morphogenetic processes, during
which they undergo highly coordinated cell shape changes. Here, we review some common …

We describe a set of replicative, integrative and single‐stranded shuttle vectors constructed
from the pUC19 plasmid that we use routinely in our experiments. They bear a yeast …

Polarized intracellular trafficking in epithelia is critical in development, immunity, and physiology
to deliver morphogens, defensins, or ion pumps to the appropriate membrane domain. …

Mechanotransduction refers to the transformation of physical forces into chemical signals. It
generally involves stretch-sensitive channels or conformational change of cytoskeleton-…

Exosomes are secreted vesicles arising from the fusion of multivesicular bodies (MVBs) with
the plasma membrane. Despite their importance in various processes, the molecular …

To build complex organs, embryos have evolved mechanisms that integrate the development
of cells unrelated to one another by cell type or ancestry. Here we show that the pha-4 …

Specialised subapical junctions play a critical role in maintaining epithelial cell polarity and
tissue integrity, and provide a platform for intracellular signalling. Here we analyse the roles …

Epithelial cells are polarized, with apical and basal compartments demarcated by tight and
adherens junctions. Proper establishment of these subapical junctions is critical for normal …