Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …

The specific and covalent labeling of the protein HaloTag with fluorescent probes in living
cells makes it a powerful tool for bioimaging. However, the irreversible attachment of the probe …

Coenzyme A (CoA) is one of the central cofactors of metabolism, yet a method for measuring
its concentration in living cells is missing. Here we introduce the first biosensor for …

Self-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins
with synthetic fluorophores for use in fluorescence microscopy. Here we introduce HaloTag …

Photoactivatable fluorophores are important for single-particle tracking and super-resolution
microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon …

Fluorescence lifetime multiplexing requires fluorescent probes with distinct fluorescence
lifetimes but similar spectral properties. Even though synthetic probes for many cellular targets …

Fluorescent biosensors enable the study of cell physiology with spatiotemporal resolution;
yet, most biosensors suffer from relatively low dynamic ranges. Here, we introduce a family of …

Genetically encoded fluorescent biosensors have revolutionized the study of cell signaling
and metabolism, as they allow for live-cell measurements with high spatiotemporal resolution. …

Genetically encoded fluorescent calcium indicators have revolutionized neuroscience and
other biological fields by allowing cellular-resolution recording of physiology during behavior. …

Malaria remains a major threat to mankind due to the perpetual emergence of resistance
against marketed drugs. Twenty‐one pyrazolopyran‐based inhibitors bearing terminal biphenyl…