▪ Abstract Chromosomal DNA replicases are multicomponent machines that have evolved
clever strategies to perform their function. Although the structure of DNA is elegant in its …

The crystal structure of the p subunit (processivity factor) of DNA polymerase Ill holoenzyme
has been determined at 2.5 A resolution. A dimer of the p subunit (M,= 2 x 40.6 kd, 2 x 366 …

The crystal structure of the human DNA polymerase δ processivity factor PCNA (proliferating
cell nuclear antigen) complexed with a 22 residue peptide derived from the C-terminus of …

The damage-inducible UmuD′ and UmuC proteins are required for most SOS mutagenesis
in Escherichia coli. Our recent assay to reconstitute this process in vitro, using a native …

DNA polymerase III holoenzyme contains two DNA polymerases embedded in a particle with
9 other subunits. This multi subunit DNA polymerase is the Escherichia coli chromosomal …

The expression of the Escherichia coli DNA polymerases pol V (UmuD′ 2 C complex) 1 , 2
and pol IV (DinB) 3 increases in response to DNA damage 4 . The induction of pol V is …

Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on
DNA polymerases. Here we report the crystal structure of the five-protein clamp loader …

DNA polymerase III holoenzyme (holoenzyme), the multiprotein replicase of Escherichia coli,
is essentially unlimited in processive DNA synthesis. Processive activity can be …

The γ complex, an AAA+ ATPase, is the bacterial homolog of eukaryotic replication factor C (RFC)
that loads the sliding clamp (β, homologous to PCNA) onto DNA. The 2.7/3.0 Å crystal …

In this era of rapid and unsettling change, boys now more than ever face difficulties in
establishing their self-image and status. In this original and challenging study Mike O'Donnell and …