Persistent latent reservoir of replication-competent proviruses in memory CD4 T cells is a
major obstacle to curing HIV infection. Pharmacological activation of HIV expression in latently …

Retroviruses utilize cellular dNTPs to perform proviral DNA synthesis in infected host cells.
Unlike oncoretroviruses, which replicate in dividing cells, lentiviruses, such as human …

A high-throughput platform would greatly facilitate coronavirus disease 2019 (COVID-19)
serological testing and antiviral screening. Here we present a high-throughput nanoluciferase …

HIV-1 integrase (IN) is the target for two classes of antiretrovirals: i) the integrase strand-transfer
inhibitors (INSTIs) and ii) the non-catalytic site integrase inhibitors (NCINIs). NCINIs bind …

HIV reservoirs and production of viral antigens are not eliminated in chronically infected
participants treated with combination antiretroviral therapy (cART). Novel therapeutic strategies …

Pyrimidinol carboxylic acids were designed as inhibitors of HIV-1 RNase H function. These
molecules can coordinate to two divalent metal ions in the RNase H active site. Inhibition of …

HIV-1 RNase H breaks down the intermediate RNA-DNA hybrids during reverse
transcription, requiring two divalent metal ions for activity. Pyrimidinol carboxylic acid and N-hydroxy …

Potentiometric stripping analysis is employed for highly sensitive measurements of single‐stranded
DNA at carbon paste electrodes. Electrochemical (oxidative) pretreatment is used to …

The role of RNA-RNA template interactions in facilitating recombination during reverse
transcription of minus strand DNA has been examined. The tested hypothesis is that template …

Recombination promotes retrovirus evolution. It involves transferring a growing DNA primer
from one genomic RNA template in the virus to the other. Strand transfer results in vitro …