CRISPR/Cas9 is a programmable genome editing tool widely used for biological applications
and engineered Cas9s have increased discrimination against off-target cleavage …

CRISPR–Cas9 as a programmable genome editing tool is hindered by off-target DNA cleavage
1 , 2 , 3 – 4 , and the underlying mechanisms by which Cas9 recognizes mismatches are …

The mechanism of DNA polymerase (pol) fidelity is of fundamental importance in chemistry
and biology. While high-fidelity pols have been well studied, much less is known about how …

Bacterial adaptive immune systems employ clustered regularly interspaced short palindromic
repeats (CRISPR) along with their CRISPR-associated genes (Cas) to form CRISPR RNA (…

It was reported in 1995 that T7 and Taq DNA polymerases possess 3′-esterase activity,
but without follow-up studies. Here we report that the 3′-esterase activity is intrinsic to the …

Methyltransferases play crucial roles in many cellular processes, and various regulatory
mechanisms have evolved to control their activities. For methyltransferases involved in …

In the process of urbanization in China, the problem of atmospheric suspended particulate
matter pollution is becoming increasingly serious. It has been impossible to completely rely …

Publisher Correction: Structural basis for mismatch surveillance by CRISPR–Cas9 - PMC Back
to Top Skip to main content NIH NLM Logo Access keys NCBI Homepage MyNCBI Homepage …

Aspirin has positive effects on bone marrow mesenchymal stem cells (BMSCs) osteogenic
differentiation. However, researchers did not give much thought to its effect on BMSCs …

To satisfy the service requirements of wave-absorbing materials in harsh environments, it is
important to design and manufacture corrosion-resistant composite-absorbing materials. …