Mitochondrial quality control pathways as determinants of metabolic health

NM Held, RH Houtkooper - Bioessays, 2015 - Wiley Online Library
Mitochondrial function is key for maintaining cellular health, while mitochondrial failure is
associated with various pathologies, including inherited metabolic disorders and age‐related …

Tetracycline antibiotics impair mitochondrial function and its experimental use confounds research

IA Chatzispyrou, NM Held, L Mouchiroud, J Auwerx… - Cancer research, 2015 - AACR
Tetracyclines, a class of antibiotics that target bacterial translation, are commonly used in
research for inducible gene expression using Tet-ON/Tet-OFF systems. However, such …

[HTML][HTML] Quercetin lowers plasma triglycerides accompanied by white adipose tissue browning in diet-induced obese mice

EN Kuipers, AD Dam, NM Held, IM Mol… - International journal of …, 2018 - mdpi.com
Obesity and dyslipidemia are major risk factors for the development of cardiovascular
diseases (CVD). Quercetin, a natural flavonoid, lowers plasma triglycerides (TG) in human …

[HTML][HTML] Barth syndrome cells display widespread remodeling of mitochondrial complexes without affecting metabolic flux distribution

IA Chatzispyrou, S Guerrero-Castillo, NM Held… - … et Biophysica Acta (BBA …, 2018 - Elsevier
… E1 activity was measured spectrophotometrically at 340 nm … was followed in time at 340 nm.
CS activity was measured using … the increase in absorbance was followed in time at 412 nm. …

[HTML][HTML] Pyruvate dehydrogenase complex plays a central role in brown adipocyte energy expenditure and fuel utilization during short-term beta-adrenergic activation

NM Held, EN Kuipers, M van Weeghel… - Scientific reports, 2018 - nature.com
… For differentiation, cells were kept at complete confluency and after two days 2 nM triiodothyronine
(Sigma-Aldrich) and 2 µM insulin (Sigma-Aldrich) was added to the medium for 9 days…

A single day of high-fat diet feeding induces lipid accumulation and insulin resistance in brown adipose tissue in mice

EN Kuipers, NM Held… - American Journal …, 2019 - journals.physiology.org
… *EN Kuipers and NM Held contributed equally to this work. … *EN Kuipers and NM Held
contributed equally to this work. … * EN Kuipers and NM Held contributed equally to this work. …

[HTML][HTML] Skeletal muscle in healthy humans exhibits a day-night rhythm in lipid metabolism

NM Held, J Wefers, M van Weeghel, S Daemen… - Molecular …, 2020 - Elsevier
… resulting in a pixel size of 81.95 nm by 81.95 nm. Laminin, myosin heavy chain type I and
Bodipy 493/503 were imaged using the 405 nm, 532 nm, and 488 nm laser lines, respectively. …

[HTML][HTML] Deletion of NLRX1 increases fatty acid metabolism and prevents diet-induced hepatic steatosis and metabolic syndrome

…, E Rampanelli, G Stokman, LM Butter, NM Held… - … et Biophysica Acta (BBA …, 2018 - Elsevier
… Cytochrome c reduction was measured at 550 nm using a ClarioSTAR plate reader. Data …
(K) Steatosis increase after 24 h 50 μM palmitate conditioned medium + 100 nM AntimycinA …

[HTML][HTML] The antibiotic doxycycline impairs cardiac mitochondrial and contractile function

RCI Wüst, BF Coolen, NM Held, MRR Daal… - International journal of …, 2021 - mdpi.com
… , Milton, MA, USA) was used for rapid wavelength switching between 340 and 380 nm.
Excitation was measured at 510 nm at 250 Hz. The F340/F380 ratio was used as a measure of …

[HTML][HTML] Aging selectively dampens oscillation of lipid abundance in white and brown adipose tissue

NM Held, MR Buijink, HL Elfrink, S Kooijman… - Scientific reports, 2021 - nature.com
Lipid metabolism is under the control of the circadian system and circadian dysregulation
has been linked to obesity and dyslipidemia. These factors and outcomes have also been …