End-resection at DNA double-strand breaks in the three domains of life

JK Blackwood, NJ Rzechorzek, SM Bray… - Biochemical Society …, 2013 - portlandpress.com
During DNA repair by HR (homologous recombination), the ends of a DNA DSB (double-strand
break) must be resected to generate single-stranded tails, which are required for strand …

[PDF][PDF] Crystal structure of the rad9-rad1-hus1 DNA damage checkpoint complex—implications for clamp loading and regulation

AS Doré, ML Kilkenny, NJ Rzechorzek, LH Pearl - Molecular cell, 2009 - cell.com
Rad9, Rad1, and Hus1 form a heterotrimeric complex (9-1-1) that is loaded onto DNA at sites
of DNA damage. DNA-loaded 9-1-1 activates signaling through the Chk1 arm of the DNA …

CryoEM structures of human CMG–ATPγS–DNA and CMG–AND-1 complexes

NJ Rzechorzek, SW Hardwick… - Nucleic acids …, 2020 - academic.oup.com
DNA unwinding in eukaryotic replication is performed by the Cdc45–MCM–GINS (CMG)
helicase. Although the CMG architecture has been elucidated, its mechanism of DNA unwinding …

CtIP tetramer assembly is required for DNA-end resection and repair

…, M Demir, CR Morton, NJ Rzechorzek… - Nature structural & …, 2015 - nature.com
Mammalian CtIP protein has major roles in DNA double-strand break (DSB) repair. Although
it is well established that CtIP promotes DNA-end resection in preparation for homology-…

[HTML][HTML] Mechanism of substrate hydrolysis by the human nucleotide pool sanitiser DNPH1

NJ Rzechorzek, S Kunzelmann, AG Purkiss… - Nature …, 2023 - nature.com
Poly(ADP-ribose) polymerase (PARP) inhibitors are used in the clinic to treat BRCA-deficient
breast, ovarian and prostate cancers. As their efficacy is potentiated by loss of the …

[HTML][HTML] Structure of the hexameric HerA ATPase reveals a mechanism of translocation-coupled DNA-end processing in archaea

NJ Rzechorzek, JK Blackwood, SM Bray… - Nature …, 2014 - nature.com
The HerA ATPase cooperates with the NurA nuclease and the Mre11–Rad50 complex for the
repair of double-strand DNA breaks in thermophilic archaea. Here we extend our structural …

Structural and functional insights into DNA-end processing by the archaeal HerA helicase–NurA nuclease complex

JK Blackwood, NJ Rzechorzek, AS Abrams… - Nucleic acids …, 2012 - academic.oup.com
Helicase–nuclease systems dedicated to DNA end resection in preparation for homologous
recombination (HR) are present in all kingdoms of life. In thermophilic archaea, the HerA …

Structural basis for the interaction of SARS‐CoV‐2 virulence factor nsp1 with DNA polymerase α–primase

…, SW Hardwick, DY Chirgadze, NJ Rzechorzek… - Protein …, 2022 - Wiley Online Library
The molecular mechanisms that drive the infection by the severe acute respiratory
syndrome coronavirus 2 (SARS‐CoV‐2)—the causative agent of coronavirus disease 2019 (COVID‐…

Structural basis for inhibition of human primase by arabinofuranosyl nucleoside analogues fludarabine and vidarabine

S Holzer, NJ Rzechorzek, IR Short… - ACS Chemical …, 2019 - ACS Publications
Nucleoside analogues are widely used in clinical practice as chemotherapy drugs. Arabinose
nucleoside derivatives such as fludarabine are effective in the treatment of patients with …

Structural basis for the coiled-coil architecture of human CtIP

CR Morton, NJ Rzechorzek, JD Maman… - Open …, 2021 - royalsocietypublishing.org
The DNA repair factor CtIP has a critical function in double-strand break (DSB) repair by
homologous recombination, promoting the assembly of the repair apparatus at DNA ends and …