Despite decades of accumulated knowledge about proteins and their post-translational
modifications (PTMs), numerous questions remain regarding their molecular composition and …

… A heterogeneous 42-kDa protein was found to have a ragged N-terminus and an
alkylated Cys residue in its active site (18). Analysis of a cluster of recombinant gene …

The rise of the “Top Down” method in the field of mass spectrometry-based proteomics has
ushered in a new age of promise and challenge for the characterization and identification of …

Neutralization-reionization mass spectrometry (MS) 1 is of unique value for preparing and
characterizing highly reactive and unstable neutral species, such as the intermediate in the …

For proteins of <20 kDa, this new radical site dissociation method cleaves different and many
more backbone bonds than the conventional MS/MS methods (eg, collisionally activated …

The EZH2 histone methyltransferase is highly expressed in germinal center (GC) B cells
and targeted by somatic mutations in B cell lymphomas. Here, we find that EZH2 deletion or …

A surprise revealed by the success of the human genome project was the lower-than-anticipated
number of genes identified:∼ 20,300, rather than the∼ 100,000 estimated 1. This …

A full description of the human proteome relies on the challenging task of detecting mature
and changing forms of protein molecules in the body. Large-scale proteome analysis 1 has …

… For K 17 −N, 31 the bond cleavages H 16 /K 17 and N 31 /I 32 are not involved in the
formation of any other peptide. The I 208 −L 222 assignment was confirmed by MS/MS that …

… Zabrouskov, V., Senko, MW, Du, Y., LeDuc, RD & Kelleher, NL New and automated MS n
approaches for top down identification of modified proteins. J. Am. … Neil L Kelleher …