[HTML][HTML] Interactions among cytochromes P450 in microsomal membranes: oligomerization of cytochromes P450 3A4, 3A5, and 2E1 and its functional consequences
DR Davydov, NY Davydova, EV Sineva… - Journal of Biological …, 2015 - ASBMB
The body of evidence of physiologically relevant P450-P450 interactions in microsomal
membranes continues to grow. Here we probe oligomerization of human CYP3A4, CYP3A5, and …
membranes continues to grow. Here we probe oligomerization of human CYP3A4, CYP3A5, and …
[HTML][HTML] Peripheral ligand-binding site in cytochrome P450 3A4 located with fluorescence resonance energy transfer (FRET)
…, EV Sineva, H Fernando, NY Davydova… - Journal of Biological …, 2012 - ASBMB
The mechanisms of ligand binding and allostery in the major human drug-metabolizing
enzyme cytochrome P450 3A4 (CYP3A4) were explored with fluorescence resonance energy …
enzyme cytochrome P450 3A4 (CYP3A4) were explored with fluorescence resonance energy …
Pivotal role of P450–P450 interactions in CYP3A4 allostery: the case of α-naphthoflavone
DR Davydov, NY Davydova, EV Sineva… - Biochemical …, 2013 - portlandpress.com
We investigated the relationship between oligomerization of CYP3A4 (cytochrome P450
3A4) and its response to ANF (α-naphthoflavone), a prototypical heterotropic activator. The …
3A4) and its response to ANF (α-naphthoflavone), a prototypical heterotropic activator. The …
Toward a systems approach to cytochrome P450 ensemble: interactions of CYP2E1 with other P450 species and their impact on CYP1A2
NY Davydova, B Dangi, MA Maldonado… - Biochemical …, 2019 - portlandpress.com
In this study, we investigate the ability of ethanol-inducible CYP2E1 to interact with other
cytochrome P450 species and affect the metabolism of their substrates. As a model system, we …
cytochrome P450 species and affect the metabolism of their substrates. As a model system, we …
Mechanism of interactions of α-naphthoflavone with cytochrome P450 3A4 explored with an engineered enzyme bearing a fluorescent probe
TN Tsalkova, NY Davydova, JR Halpert… - Biochemistry, 2007 - ACS Publications
Design of a partially cysteine-depleted C98S/C239S/C377S/C468A cytochrome P450 3A4
mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive …
mutant designated CYP3A4(C58,C64) allowed site-directed incorporation of thiol-reactive …
[HTML][HTML] Electron transfer in the complex of membrane-bound human cytochrome P450 3A4 with the flavin domain of P450BM-3: the effect of oligomerization of the …
We studied the kinetics of NADPH-dependent reduction of human CYP3A4 incorporated
into Nanodiscs (CYP3A4–ND) and proteoliposomes in order to probe the effect of P450 …
into Nanodiscs (CYP3A4–ND) and proteoliposomes in order to probe the effect of P450 …
Kinetic mechanism of time-dependent inhibition of CYP2D6 by 3, 4-methylenedioxymethamphetamine (MDMA): functional heterogeneity of the enzyme and the …
JT Rodgers, NY Davydova, EM Paragas… - Biochemical …, 2018 - Elsevier
We investigate the mechanism of time-dependent inhibition (TDI) of human cytochrome
P450 2D6 (CYP2D6) by 3,4-methylenedioxymethamphetamine (MDMA, ecstasy), one of the …
P450 2D6 (CYP2D6) by 3,4-methylenedioxymethamphetamine (MDMA, ecstasy), one of the …
Toward a systems approach to the human cytochrome P450 ensemble: interactions between CYP2D6 and CYP2E1 and their functional consequences
DR Davydov, NY Davydova, JT Rodgers… - Biochemical …, 2017 - portlandpress.com
Functional cross-talk among human drug-metabolizing cytochrome P450 through their
association is a topic of emerging importance. Here, we studied the interactions of human …
association is a topic of emerging importance. Here, we studied the interactions of human …
Effect of glutathione on homo-and heterotropic cooperativity in cytochrome P450 3A4
DR Davydov, NY Davydova, TN Tsalkova… - Archives of biochemistry …, 2008 - Elsevier
Glutathione (GSH) exerted a profound effect on the oxidation of 7-benzyloxy-4-(trifluoromethyl)coumarin
(BFC) and 7-benzyloxyquinoline (BQ) by human liver microsomes as well as by …
(BFC) and 7-benzyloxyquinoline (BQ) by human liver microsomes as well as by …
Effects of alcohol-induced increase in CYP2E1 content in human liver microsomes on the activity and cooperativity of CYP3A4
B Dangi, NY Davydova, MA Maldonado… - Archives of biochemistry …, 2021 - Elsevier
We investigate the effect of the alcohol-induced increase in the content of CYP2E1 in human
liver microsomes (HLM) on the function of CYP3A4. Membrane incorporation of the purified …
liver microsomes (HLM) on the function of CYP3A4. Membrane incorporation of the purified …