Ribonucleoprotein (RNP) granules play an important role in organizing eukaryotic mRNA
metabolism via liquid-liquid phase separation (LLPS) of mRNA decay factors into membrane-…

Long non-coding (lnc)RNAs, once thought to merely represent noise from imprecise transcription
initiation, have now emerged as major regulatory entities in all eukaryotes. In contrast …

The transcription cycle of RNAPII is comprised of three consecutive steps; initiation, elongation
and termination. It has been assumed that the initiation and termination steps occur in …

Biomolecular condensates play a key role in organizing RNAs and proteins into
membraneless organelles. Bacterial RNP-bodies (BR-bodies) are a biomolecular condensate …

Gene looping, defined as the interaction of the promoter and the terminator regions of a gene
during transcription, requires transcription factor IIB (TFIIB). We have earlier demonstrated …

The Cleavage Factor 1A (CF1A) complex, which is required for the termination of transcription
in budding yeast, occupies the 3′ end of transcriptionally active genes. We recently …

Bacterial cell division is the result of a productive round of the cell cycle to yield two daughter
cells. The cell cycle is highly coordinated in Caulobacter crescentus where it is driven by a …

RNase E is the most common RNA decay nuclease in bacteria, setting the global mRNA
decay rate and scaffolding formation of the RNA degradosome complex and BR-bodies. To …

Bacterial RNP bodies (BR bodies) contain the mRNA decay machinery, but the collection of
associated RNAs and proteins are poorly defined. Here, we present a protocol for the rapid …

Biomolecular condensates, such as the nucleoli or P-bodies, are non-membrane-bound
assemblies of proteins and nucleic acids that facilitate specific cellular processes. Like …