The RNA-guided CRISPR–Cas9 nuclease has revolutionized genome engineering, yet its
mechanism for DNA target selection is not fully understood. A crucial step in Cas9 target …

Cas12a (also known as “Cpf1”) is a class 2 type VA CRISPR-associated nuclease that can
cleave double-stranded DNA at specific sites. The Cas12a effector enzyme comprises a …

The technique of site-directed spin labeling (SDSL) provides unique information on
biomolecules by monitoring the behavior of a stable radical tag (ie, spin label) using electron …

In a type II clustered regularly interspaced short palindromic repeats (CRISPR) system, RNAs
that are encoded at the CRISPR locus complex with the CRISPR-associated (Cas) protein …

CRISPR-Cas9 is an RNA-guided nuclease that has been widely adapted for genome
engineering. A key determinant in Cas9 target selection is DNA duplex unwinding to form an R-…

The technique of site-directed spin labeling (SDSL) provides information on bio-molecular
systems by monitoring the behaviors of a stable radical tag (ie, spin label) using electron …

A thorough understanding of the oxidation chemistry of cycloalkanes is integral to the
development of alternative fuels and improving current fuel performance. An important class of …

High throughput DNA sequencing technologies have undergone tremendous development
over the past decade. Although optical detection-based sequencing has constituted the …

Next Generation Sequencing (NGS) technologies have revolutionized basic biological and
clinical research, especially in oncology. NGS cancer assays are used to determine cancer …

CRISPR-Cas9 is an RNA-guided nuclease that has been adapted to cleave double-stranded
DNA at desired locations within eukaryote genomes. Cas9-based technology has led to a …