Many cellular activities in bacteria are organized according to their growth rate. The notion
that ppGpp measures the cell’s growth rate is well accepted in the field of bacterial physiology…

The field of optimal experimental design uses mathematical techniques to determine
experiments that are maximally informative from a given experimental setup. Here we apply a …

Recently developed dynamic experimental techniques offer new opportunities for the use of
model-based experimental design in the construction and refinement of predictive models of …

Synthetic biology design challenges have driven the use of mathematical models to characterize
genetic components and to explore complex design spaces. Traditional approaches to …

Accurate model calibration is essential for model-based design of synthetic gene regulatory
networks. Optimal experimental design (OED) techniques can be used to efficiently …

Microbial gene expression is a comparatively well understood process, but regulatory
interactions between genes can give rise to complicated behaviours. Regulatory networks can …

During environmental changes such as diauxic shifts, E. coli responds by producing ppGpp (31).
Fig. 1A shows a typical diauxic growth curve in minimal medium containing glycerol …

Modeling in systems and synthetic biology relies on accurate parameter estimates and
predictions. Accurate model calibration relies, in turn, on data and on how well suited the …

Prostate cancer is the most common non-dermatological cancer amongst men in the developed
world. The disease is manageable if detected early; treatment is thus becoming highly …

Rod-shaped bacteria such as E. coli reproduce by expanding along their long axis and
splitting into pairs of daughter cells. If conditions are favourable for growth, they will continue in …