Over the past decade, a growing community of researchers has emerged around the use of
constraint-based reconstruction and analysis (COBRA) methods to simulate, analyze and …

Chinese hamster ovary (CHO)–derived cell lines are the preferred host cells for the
production of therapeutic proteins. Here we present a draft genomic sequence of the CHO-K1 …

Cell–cell interactions orchestrate organismal development, homeostasis and single-cell
functions. When cells do not properly interact or improperly decode molecular messages, …

Genome-scale metabolic models are mathematically-structured knowledge bases that can
be used to predict metabolic pathway usage and growth phenotypes. Furthermore, they can …

Escher is a web application for visualizing data on biological pathways. Three key features
make Escher a uniquely effective tool for pathway visualization. First, users can rapidly …

Constraint-based reconstruction and analysis (COBRA) provides a molecular mechanistic
framework for integrative analysis of experimental molecular systems biology data and …

Reconstructed microbial metabolic networks facilitate a mechanistic description of the
genotype–phenotype relationship through the deployment of constraint-based reconstruction and …

Carbon fixation is the process by which CO 2 is incorporated into organic compounds. In
modern agriculture in which water, light, and nutrients can be abundant, carbon fixation could …

After hundreds of generations of adaptive evolution at exponential growth, Escherichia coli
grows as predicted using flux balance analysis (FBA) on genome‐scale metabolic models (…

Chinese hamster ovary (CHO) cells, first isolated in 1957, are the preferred production host
for many therapeutic proteins. Although genetic heterogeneity among CHO cell lines has …