[HTML][HTML] Methods matter: standard production platforms for recombinant AAV produce chemically and functionally distinct vectors

NG Rumachik, SA Malaker, N Poweleit… - … therapy Methods & …, 2020 - cell.com
Different approaches are used in the production of recombinant adeno-associated virus (rAAV).
The two leading approaches are transiently transfected human HEK293 cells and live …

Chemical Modulation of Protein O-GlcNAcylation via OGT Inhibition Promotes Human Neural Cell Differentiation

…, IW Blong, AC Evans, NG Rumachik… - ACS chemical …, 2017 - ACS Publications
The enzymes that determine protein O-GlcNAcylation, O-GlcNAc transferase (OGT) and O-GlcNAcase
(OGA), act on key transcriptional and epigenetic regulators, and both are …

Analysis of the acidic proteome with negative electron-transfer dissociation mass spectrometry

GC McAlister, JD Russell, NG Rumachik… - Analytical …, 2012 - ACS Publications
We describe the first implementation of negative electron-transfer dissociation (NETD) on a
hybrid ion trap-orbitrap mass spectrometer and its application to high-throughput sequencing …

Characterizing peptide neutral losses induced by negative electron-transfer dissociation (NETD)

NG Rumachik, GC McAlister, JD Russell… - Journal of the …, 2012 - ACS Publications
We implemented negative electron-transfer dissociation (NETD) on a hybrid ion trap/Orbitrap
mass spectrometer to conduct ion/ion reactions using peptide anions and radical reagent …

[HTML][HTML] VectorMOD: Method for bottom-up proteomic characterization of rAAV capsid post-translational modifications and vector impurities

NG Rumachik, SA Malaker, NK Paulk - Frontiers in Immunology, 2021 - frontiersin.org
Progress in recombinant AAV gene therapy product and process development has advanced
our understanding of the basic biology of this critical delivery vector. The discovery of rAAV …

[HTML][HTML] Negative electron-transfer dissociation nLC-MS/MS facilitates the analysis of thousands of acidic peptides

GC McAlister, JD Russell, NG Rumachik… - Analytical …, 2012 - ncbi.nlm.nih.gov
We describe the first implementation of negative electron-transfer dissociation (NETD) on a
hybrid ion trap-orbitrap mass spectrometer and its application to high-throughput sequencing …

[BOOK][B] Applications of Chemical Biology and Mass Spectrometry for the Analysis of the O-GlcNAcome and Adeno-Associated Viruses

NG Rumachik - 2018 - search.proquest.com
Proteins are involved in nearly all cellular processes and functions. Though there are some
20000 protein-coding genes in the human genome, the functional space of these proteins is …

[PDF][PDF] Elucidating the Complexities of the Human Milk Glycome with Ion Chromatography-Mass Spectrometry (IC-MS)

T Tian, NG Rumachik, Y Liu, C Pohl - lcms.cz
RESULTS High Performance Anion Exchange Chromatography (HPAE) has long been used
to separate complex carbohydrates. High resolution separation of chemically similar but …

Structural Characterization of Oligosaccharides in Infant Formula Products Supplemented with Prebiotics Using Ion Chromatography-Mass Spectrometry

NG Rumachik, T Tian, Y Liu - Available at SSRN 4484768 - papers.ssrn.com
Interest in functional oligosaccharides has grown substantially because of their profound
impact on the gut microbiome. As prebiotics, the health benefits of functional oligosaccharides …

Towards a more complete glycome: Advances in ion chromatography-mass spectrometry (IC-MS) for improved separation and analysis of carbohydrates

N Rumachik, T Tian, Y Hou, C Saini, J Cheng… - … of Chromatography B, 2021 - Elsevier
To date, few tools are available for the analysis of the glycome without derivatization, a process
which is known to introduce issues such as differential loss of sialic acid and incomplete …