[PDF][PDF] Efficient intracellular delivery of native proteins

…, V Prasad, RJ Lebbink, H Rehmann, N Geijsen - Cell, 2015 - cell.com
Modulation of protein function is used to intervene in cellular processes but is often done
indirectly by means of introducing DNA or mRNA encoding the effector protein. Thus far, direct …

[PDF][PDF] Sox2+ adult stem and progenitor cells are important for tissue regeneration and survival of mice

…, R Bronson, S Sengupta, M Seandel, N Geijsen… - Cell stem cell, 2011 - cell.com
The transcription factor Sox2 maintains the pluripotency of early embryonic cells and regulates
the formation of several epithelia during fetal development. Whether Sox2 continues to …

Blastocyst-like structures generated solely from stem cells

…, A Van Oudenaarden, CA Van Blitterswijk, N Geijsen - Nature, 2018 - nature.com
The blastocyst (the early mammalian embryo) forms all embryonic and extra-embryonic
tissues, including the placenta. It consists of a spherical thin-walled layer, known as the …

Derivation of embryonic germ cells and male gametes from embryonic stem cells

N Geijsen, M Horoschak, K Kim, J Gribnau, K Eggan… - Nature, 2004 - nature.com
Egg and sperm cells (gametes) of the mouse are derived from a founder population of
primordial germ cells that are set aside early in embryogenesis. Primordial germ cells arise from …

The pluripotent genome in three dimensions is shaped around pluripotency factors

…, N Festuccia, EP Nora, M Welling, E Heard, N Geijsen… - Nature, 2013 - nature.com
It is becoming increasingly clear that the shape of the genome importantly influences transcription
regulation. Pluripotent stem cells such as embryonic stem cells were recently shown to …

[PDF][PDF] Cloning-free CRISPR

M Arbab, S Srinivasan, T Hashimoto, N Geijsen… - Stem cell reports, 2015 - cell.com
We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated
genomic mutation and site-specific knockin transgene creation within several …

Simple, fast and efficient iTOP-mediated delivery of CRISPR/Cas9 RNP in difficult-to-transduce human cells including primary T cells

…, A Beier, JP Gerlach, JJ Molenaar, N Geijsen… - Journal of …, 2021 - Elsevier
Niels Geijsen for providing reagents and laboratory space, and for fruitful discussions. This
Niels Geijsen is co-inventor on a patent describing the iTOP technology® (WO 2015028969…

The roles of FGF and MAP kinase signaling in the segregation of the epiblast and hypoblast cell lineages in bovine and human embryos

…, R Wubbolts, M Welling, N Geijsen… - …, 2012 - journals.biologists.com
At the blastocyst stage of mammalian pre-implantation development, three distinct cell lineages
have formed: trophectoderm, hypoblast (primitive endoderm) and epiblast. The inability …

[HTML][HTML] Hominin-specific regulatory elements selectively emerged in oligodendrocytes and are disrupted in autism patients

…, G Geeven, V Bianchi, W de Laat, N Geijsen… - Nature …, 2020 - nature.com
Speciation is associated with substantial rewiring of the regulatory circuitry underlying the
expression of genes. Determining which changes are relevant and underlie the emergence of …

[HTML][HTML] Prime editing: advances and therapeutic applications

Z Zhao, P Shang, P Mohanraju, N Geijsen - Trends in Biotechnology, 2023 - cell.com
Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR–Cas)-mediated
genome editing has revolutionized biomedical research and will likely change …