Enzymes maintain metabolism, and their concentration affects cellular fitness: high enzyme
levels are costly, and low enzyme levels can limit metabolic flux. Here, we used CRISPR …

Microbes must ensure robust amino acid metabolism in the face of external and internal
perturbations. This robustness is thought to emerge from regulatory interactions in metabolic …

Synthetic metabolic pathways are a burden for engineered bacteria, but the underlying
mechanisms often remain elusive. Here we show that the misregulated activity of the transcription …

The operation of the central metabolism is typically assumed to be deterministic, but dynamics
and high connectivity of the metabolic network make it potentially prone to generating …

Metabolism controls gene expression through allosteric interactions between metabolites
and transcription factors. These interactions are usually measured with in vitro assays, but …

Flow-injection mass spectrometry (FI-MS) enables metabolomics studies with a very high
sample-throughput. However, FI-MS is prone to in-source modifications of analytes because …

7.1 Abstract Flow-injection mass spectrometry (FI-MS) enables metabolomics studies with a
very high sample-throughput. In most FI-MS methods, samples are directly injected into the …

Der mikrobielle Stoffwechsel ist komplex und wird durch Feedback Regulation auf vielen
Ebenen reguliert. Feedback Regulation kann durch Metabolit-Protein Interaktionen ausgeführt …

Flow-injection mass spectrometry (FI-MS) enables metabolomics studies with a very high
sample-throughput. In most FI-MS methods, samples are directly injected into the electrospray …

8.1 Abstract To identify metabolites, mass spectrometry-based metabolomics approaches rely
on accurate spectral databases that contain fragment signatures of all metabolites. These …