User profiles for Nilisha Pokhrel
 | Nilisha PokhrelMarquette University Verified email at marquette.edu Cited by 511 |
[HTML][HTML] A structural and dynamic model for the assembly of Replication Protein A on single-stranded DNA
Replication Protein A (RPA), the major eukaryotic single stranded DNA-binding protein,
binds to exposed ssDNA to protect it from nucleases, participates in a myriad of nucleic acid …
binds to exposed ssDNA to protect it from nucleases, participates in a myriad of nucleic acid …
Evidence That the Pi Release Event Is the Rate-Limiting Step in the Nitrogenase Catalytic Cycle
…, B Eilers, Q Guo, N Pokhrel… - Biochemistry, 2016 - ACS Publications
Nitrogenase reduction of dinitrogen (N 2 ) to ammonia (NH 3 ) involves a sequence of events
that occur upon the transient association of the reduced Fe protein containing two ATP …
that occur upon the transient association of the reduced Fe protein containing two ATP …
Dynamics and selective remodeling of the DNA-binding domains of RPA
N Pokhrel, CC Caldwell, EI Corless… - Nature structural & …, 2019 - nature.com
Replication protein A (RPA) coordinates important DNA metabolic events by stabilizing
single-stranded DNA (ssDNA) intermediates, activating the DNA-damage response and handing …
single-stranded DNA (ssDNA) intermediates, activating the DNA-damage response and handing …
Rapid 40 kb genome construction from 52 parts through data-optimized assembly design
Large DNA constructs (>10 kb) are invaluable tools for genetic engineering and the
development of therapeutics. However, the manufacture of these constructs is laborious, often …
development of therapeutics. However, the manufacture of these constructs is laborious, often …
[HTML][HTML] Yeast Rad52 is a homodecamer and possesses BRCA2-like bipartite Rad51 binding modes
…, S Karunakaran, MJ Rau, K Basore, N Pokhrel… - Nature …, 2023 - nature.com
Homologous recombination (HR) is an essential double-stranded DNA break repair
pathway. In HR, Rad52 facilitates the formation of Rad51 nucleoprotein filaments on RPA-coated …
pathway. In HR, Rad52 facilitates the formation of Rad51 nucleoprotein filaments on RPA-coated …
[HTML][HTML] Rtt105 regulates RPA function by configurationally stapling the flexible domains
Replication Protein A (RPA) is a heterotrimeric complex that binds to single-stranded DNA (ssDNA)
and recruits over three dozen RPA-interacting proteins to coordinate multiple …
and recruits over three dozen RPA-interacting proteins to coordinate multiple …
Monitoring Replication Protein A (RPA) dynamics in homologous recombination through site-specific incorporation of non-canonical amino acids
N Pokhrel, S Origanti, EP Davenport… - Nucleic acids …, 2017 - academic.oup.com
An essential coordinator of all DNA metabolic processes is Replication Protein A (RPA). RPA
orchestrates these processes by binding to single-stranded DNA (ssDNA) and interacting …
orchestrates these processes by binding to single-stranded DNA (ssDNA) and interacting …
[HTML][HTML] An Aurora B-RPA signaling axis secures chromosome segregation fidelity
Errors in chromosome segregation underlie genomic instability associated with cancers.
Resolution of replication and recombination intermediates and protection of vulnerable single-…
Resolution of replication and recombination intermediates and protection of vulnerable single-…
[HTML][HTML] Phosphate removal and recovery using immobilized phosphate binding proteins
Progress towards a more circular phosphorus economy necessitates development of innovative
water treatment systems which can reversibly remove inorganic phosphate (P i ) to ultra-…
water treatment systems which can reversibly remove inorganic phosphate (P i ) to ultra-…
The Srs2 helicase dampens DNA damage checkpoint by recycling RPA from chromatin
The DNA damage checkpoint induces many cellular changes to cope with genotoxic stress.
However, persistent checkpoint signaling can be detrimental to growth partly due to …
However, persistent checkpoint signaling can be detrimental to growth partly due to …