Massively parallel sequencing of cDNA has enabled deep and efficient probing of transcriptomes.
Current approaches for transcript reconstruction from such data often rely on aligning …

INTRODUCTION Dendritic cells (DCs) and monocytes consist of multiple specialized
subtypes that play a central role in pathogen sensing, phagocytosis, and antigen presentation. …

Abstract Treatment with immune checkpoint blockade (ICB) has revolutionized cancer therapy.
Until now, predictive biomarkers 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 – 10 and strategies to augment …

Treatment of cancer has been revolutionized by immune checkpoint blockade therapies.
Despite the high rate of response in advanced melanoma, the majority of patients succumb to …

Crucial transitions in cancer—including tumor initiation, local expansion, metastasis, and
therapeutic resistance—involve complex interactions between cells within the dynamic tumor …

CRISPR-Cas9 is a versatile genome editing technology for studying the functions of genetic
elements. To broadly enable the application of Cas9 in vivo, we established a Cre-…

The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form
The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens …

Recent molecular studies have shown that, even when derived from a seemingly homogenous
population, individual cells can exhibit substantial differences in gene expression, protein …

Cancer genome sequencing has enabled the rapid identification of the complete repertoire
of coding sequence mutations within a patient's tumor and facilitated their use as …

How the genomic landscape of a tumor shapes and is shaped by anti-tumor immunity has
not been systematically explored. Using large-scale genomic data sets of solid tissue tumor …