Long-read sequencing (LRS) has become increasingly popular due to its strengths in de
novo assembly and in resolving complex DNA regions as well as in determining full-length …

In this study, we used the amplified isoform sequencing technique from Pacific Biosciences
to characterize the poly(A) + fraction of the lytic transcriptome of the herpes simplex virus type …

Background Varicella zoster virus (VZV) is a human pathogenic alphaherpesvirus harboring
a relatively large DNA molecule. The VZV transcriptome has already been analyzed by …

Third-generation sequencing is an emerging technology that is capable of solving several
problems that earlier approaches were not able to, including the identification of transcripts …

The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an insect-pathogen
baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the …

Herpesvirus gene expression is co-ordinately regulated and sequentially ordered during
productive infection. The viral genes can be classified into three distinct kinetic groups: …

The role of RNA molecules in the priming of DNA replication and in providing a template for
telomerase extension has been known for decades. Since then, several transcripts have …

Long-read sequencing (LRS) has become increasingly important in RNA research due to its
strength in resolving complex transcriptomic architectures. In this regard, currently two LRS …

Background Alternative polyadenylation is commonly examined using cDNA sequencing,
which is known to be affected by template-switching artifacts. However, the effects of such …

In this study, we used Pacific Biosciences RS II long-read and Illumina HiScanSQ short-read
sequencing technologies for the characterization of porcine circovirus type 1 (PCV-1) …