Diurnal metabolic control in cyanobacteria requires perception of second messenger signaling molecule c-di-AMP by the carbon control protein SbtB

KA Selim, M Haffner, M Burkhardt, O Mantovani… - Science …, 2021 - science.org
Because of their photosynthesis-dependent lifestyle, cyanobacteria evolved sophisticated
regulatory mechanisms to adapt to oscillating day-night metabolic changes. How they …

The impact of the cyanobacterial carbon‐regulator protein SbtB and of the second messengers cAMP and c‐di‐AMP on CO2‐dependent gene expression

O Mantovani, V Reimann, M Haffner… - New …, 2022 - Wiley Online Library
The amount of inorganic carbon (C i ) fluctuates in aquatic environments. Cyanobacteria
evolved a C i ‐concentrating mechanism (CCM) that is regulated at different levels. The …

[HTML][HTML] High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803

D Dienst, J Wichmann, O Mantovani, JS Rodrigues… - Scientific reports, 2020 - nature.com
Cyanobacteria and microalgae are attractive photoautotrophic host systems for climate-friendly
production of fuels and other value-added biochemicals. However, for economic …

Red/far-red light signals regulate the activity of the carbon-concentrating mechanism in cyanobacteria

N Oren, S Timm, M Frank, O Mantovani, O Murik… - Science …, 2021 - science.org
Desiccation-tolerant cyanobacteria can survive frequent hydration/dehydration cycles likely
affecting inorganic carbon (Ci) levels. It was recently shown that red/far-red light serves as …

Carbon signaling protein SbtB possesses atypical redox-regulated apyrase activity to facilitate regulation of bicarbonate transporter SbtA

KA Selim, M Haffner, O Mantovani… - Proceedings of the …, 2023 - National Acad Sciences
The PII superfamily consists of widespread signal transduction proteins found in all domains
of life. In addition to canonical PII proteins involved in C/N sensing, structurally similar PII-…

[HTML][HTML] Roles of second messengers in the regulation of cyanobacterial physiology: the carbon-concentrating mechanism and beyond

O Mantovani, M Haffner, KA Selim, M Hagemann… - …, 2023 - academic.oup.com
Second messengers are a fundamental category of small molecules and ions that are involved
in the regulation of many processes in all domains of life. Here we focus on cyanobacteria…

The redox-sensitive R-loop of the carbon control protein SbtB contributes to the regulation of the cyanobacterial CCM

O Mantovani, M Haffner, P Walke, AA Elshereef… - 2023 - researchsquare.com
SbtB is a PII-like protein that regulates the carbon-concentrating mechanism (CCM) in
cyanobacteria. SbtB proteins can bind many adenyl nucleotides and possess a characteristic C-…

Diurnal rhythm causes metabolic crises in the cyanobacterial mutants of c-di-AMP signalling cascade

M Haffner, O Mantovani, P Spaet, B Macek… - bioRxiv, 2023 - biorxiv.org
In nature, the photoautotrophic lifestyle of cyanobacteria has to cope with the successive
diurnal changes in light supply. Light supply throughout the day enables photosynthesis and …

PII signal transduction superfamily acts as a valve plug to control bicarbonate and ammonia homeostasis among different bacterial phyla

M Haffner, WT Hou, O Mantovani, PR Walke, K Hauf… - bioRxiv, 2023 - biorxiv.org
Life on Earth relies on carbon and nitrogen assimilation by RubisCO and GS-GOGAT enzymes,
respectively, whose activities depend on a constant supply of inorganic carbon (Ci) and …

High Density Cultivation of Synechocystis sp. PCC 6803 using the HDC 6.10 B system (CellDeg)

O Mantovani, D Dienst, P Lindberg - 2019 - protocols.io
The CellDeg© high density cultivation system is a revolutionary culturing system for
photoautotrophic microorganisms. The culturing vessels come with a semi-permeable membrane on …