[HTML][HTML] Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system
B Zetsche, JS Gootenberg, OO Abudayyeh… - cell, 2015 - cell.com
The microbial adaptive immune system CRISPR mediates defense against foreign genetic
elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi…
elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi…
Nucleic acid detection with CRISPR-Cas13a/C2c2
Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen
detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered …
detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered …
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Systematic interrogation of gene function requires the ability to perturb gene expression in a
robust and generalizable manner. Here we describe structure-guided engineering of a …
robust and generalizable manner. Here we describe structure-guided engineering of a …
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
INTRODUCTION Almost all archaea and about half of bacteria possess clustered regularly
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
Rapid detection of nucleic acids is integral for clinical diagnostics and biotechnological
applications. We recently developed a platform termed SHERLOCK (specific high-sensitivity …
applications. We recently developed a platform termed SHERLOCK (specific high-sensitivity …
[HTML][HTML] CRISPR-based diagnostics
MM Kaminski, OO Abudayyeh, JS Gootenberg… - Nature Biomedical …, 2021 - nature.com
The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic intervention
and epidemiological surveillance. Diagnostics based on the detection of nucleic acids …
and epidemiological surveillance. Diagnostics based on the detection of nucleic acids …
RNA targeting with CRISPR–Cas13
RNA has important and diverse roles in biology, but molecular tools to manipulate and
measure it are limited. For example, RNA interference 1 , 2 , 3 can efficiently knockdown RNAs, …
measure it are limited. For example, RNA interference 1 , 2 , 3 can efficiently knockdown RNAs, …
RNA editing with CRISPR-Cas13
Nucleic acid editing holds promise for treating genetic disease, particularly at the RNA level,
where disease-relevant sequences can be rescued to yield functional protein products. …
where disease-relevant sequences can be rescued to yield functional protein products. …
[PDF][PDF] Discovery and functional characterization of diverse class 2 CRISPR-Cas systems
Microbial CRISPR-Cas systems are divided into Class 1, with multisubunit effector complexes,
and Class 2, with single protein effectors. Currently, only two Class 2 effectors, Cas9 and …
and Class 2, with single protein effectors. Currently, only two Class 2 effectors, Cas9 and …
[HTML][HTML] Detection of SARS-CoV-2 with SHERLOCK one-pot testing
…, JS Gootenberg, OO Abudayyeh… - … England Journal of …, 2020 - Mass Medical Soc
Detection of SARS-CoV-2 with a CRISPR-Based Test A new method for the detection of
SARS-CoV-2 combines simplified extraction of RNA with isothermal amplification and CRISPR (…
SARS-CoV-2 combines simplified extraction of RNA with isothermal amplification and CRISPR (…