Imaging of both the positions and orientations of single fluorophores, termed single-molecule
orientation-localization microscopy, is a powerful tool for the study of biochemical …

Fluorescence photons emitted by single molecules contain rich information regarding their
rotational motions, but adapting single-molecule localization microscopy (SMLM) to measure …

In soft matter, thermal energy causes molecules to continuously translate and rotate, even in
crowded environments, thereby impacting the spatial organization and function of most …

Simultaneous measurements of single-molecule positions and orientations provide critical
insight into a variety of biological and chemical processes. Various engineered point spread …

Synthetic peptides that self-assemble into cross-β fibrils are versatile building blocks for
engineered biomaterials due to their modularity and biocompatibility, but their structural and …

We report a radially and azimuthally polarized (raPol) microscope for high detection and
estimation performance in single-molecule orientation-localization microscopy (SMOLM). With …

Various techniques have been developed to measure the 2D and 3D positions and 2D and
3D orientations of fluorescent molecules with improved precision over standard …

Precisely measuring the three-dimensional position and orientation of individual fluorophores
is challenging due to the substantial photon shot noise in single-molecule experiments. …

Optical fluorescence imaging is capable of measuring both the translational and rotational
dynamics of single molecules. However, unavoidable measurement noise will result in …

Precisely measuring molecular orientation is key to understanding how molecules organize
and interact in soft matter, but the maximum theoretical limit of measurement precision has …