Ubiquitination is initiated by transfer of ubiquitin (Ub) from a ubiquitin-activating enzyme (E1)
to a ubiquitin-conjugating enzyme (E2), producing a covalently linked intermediate (E2–Ub) …

Intracellular signaling during oxidative stress is complex, with organelle-to-nucleus
retrograde communication pathways ill-defined or incomplete. Here we identify the 3′-…

The reversibility of ubiquitination by the action of deubiquitinating enzymes (DUBs) serves
as an important regulatory layer within the ubiquitin system. Approximately 100 DUBs are …

Highlights • Laboratory directed evolution and ancestral reconstruction can facilitate study of
protein structural dynamics. • Advances in biophysical and computational techniques …

Developments in computational chemistry, bioinformatics, and laboratory evolution have
facilitated the de novo design and catalytic optimization of enzymes. Besides creating useful …

MYCBP2 is a ubiquitin (Ub) E3 ligase (E3) that is essential for neurodevelopment and
regulates axon maintenance. MYCBP2 transfers Ub to nonlysine substrates via a newly …

Photosystem II (PS II) is a macromolecular complex responsible for light-driven oxidation of
water and reduction of plastoquinone as part of the photosynthetic electron transport chain …

Resisting and tolerating microbes are alternative strategies to survive infection, but little is
known about the evolutionary mechanisms controlling this balance. Here genomic analyses of …

… PD Mabbitt et al., Conformational disorganization within the active site of a recently evolved
organophosphate hydrolase limits its catalytic efficiency. Biochemistry 55, 1408–1417 (2016)…

Oligomerization has been suggested to be an important mechanism for increasing or maintaining
the thermostability of proteins. Although it is evident that protein–protein contacts can …