Class 2 CRISPR–Cas RNA-guided endonucleases: Swiss Army knives of genome editing
S Stella, P Alcon, G Montoya - Nature Structural & Molecular Biology, 2017 - nature.com
CRISPR–Cas is a bacterial defense system against phage infection and nucleic acid invasion.
Class 2 type II CRISPR–Cas9 has also been widely used for genome engineering. Here, …
Class 2 type II CRISPR–Cas9 has also been widely used for genome engineering. Here, …
[PDF][PDF] Conformational activation promotes CRISPR-Cas12a catalysis and resetting of the endonuclease activity
Cas12a, also known as Cpf1, is a type VA CRISPR-Cas RNA-guided endonuclease that is
used for genome editing based on its ability to generate specific dsDNA breaks. Here, we …
used for genome editing based on its ability to generate specific dsDNA breaks. Here, we …
Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage
S Stella, P Alcón, G Montoya - Nature, 2017 - nature.com
Cpf1 is an RNA-guided endonuclease that is emerging as a powerful genome-editing tool 1
, 2 . Here we provide insight into its DNA-targeting mechanism by determining the structure …
, 2 . Here we provide insight into its DNA-targeting mechanism by determining the structure …
FANCD2–FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair
Vertebrate DNA crosslink repair excises toxic replication-blocking DNA crosslinks.
Numerous factors involved in crosslink repair have been identified, and mutations in their …
Numerous factors involved in crosslink repair have been identified, and mutations in their …
Structure of the Fanconi anaemia monoubiquitin ligase complex
The Fanconi anaemia (FA) pathway repairs DNA damage caused by endogenous and
chemotherapy-induced DNA crosslinks, and responds to replication stress 1 , 2 . Genetic …
chemotherapy-induced DNA crosslinks, and responds to replication stress 1 , 2 . Genetic …
The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination
DNA interstrand cross-links are tumor-inducing lesions that block DNA replication and
transcription. When cross-links are detected at stalled replication forks, ATR kinase …
transcription. When cross-links are detected at stalled replication forks, ATR kinase …
[HTML][HTML] The histone chaperone SPT2 regulates chromatin structure and function in Metazoa
Histone chaperones control nucleosome density and chromatin structure. In yeast, the H3–H4
chaperone Spt2 controls histone deposition at active genes but its roles in metazoan …
chaperone Spt2 controls histone deposition at active genes but its roles in metazoan …
[HTML][HTML] PSTPIP1-LYP phosphatase interaction: structural basis and implications for autoinflammatory disorders
Mutations in the adaptor protein PSTPIP1 cause a spectrum of autoinflammatory diseases,
including PAPA and PAMI; however, the mechanism underlying these diseases remains …
including PAPA and PAMI; however, the mechanism underlying these diseases remains …
Substrate integrated waveguides structures using frequency selective surfaces operating in stop-band (SBFSS-SIW)
N Esparza, P Alcón, LF Herrán… - IEEE Microwave and …, 2016 - ieeexplore.ieee.org
… Gobierno del Principado de Asturias and FEDER (Unión Europea) under Project GRUPIN14-114,
and by the Ministerio de Economía y Competitividad under Project TEC2014-54005-P. …
and by the Ministerio de Economía y Competitividad under Project TEC2014-54005-P. …
Toxicity of blue led light and A2E is associated to mitochondrial dynamics impairment in ARPE-19 cells: implications for age-related macular degeneration
…, GG Liñares, JM Bujjamer, RM Gorojod, SP Alcon… - Archives of …, 2019 - Springer
… Experimental comparisons between treatments were made by Student’s t test or one-way
ANOVA, followed by Student-Newman–Keuls post hoc test with statistical significance set at p …
ANOVA, followed by Student-Newman–Keuls post hoc test with statistical significance set at p …