User profiles for P. Lotfy
 | Peter LotfyPhD Candidate at Harvard University, HHMI Gilliam Fellow Verified email at g.harvard.edu Cited by 1653 |
[HTML][HTML] Transcriptome engineering with RNA-targeting type VI-D CRISPR effectors
Class 2 CRISPR-Cas systems endow microbes with diverse mechanisms for adaptive
immunity. Here, we analyzed prokaryotic genome and metagenome sequences to identify an …
immunity. Here, we analyzed prokaryotic genome and metagenome sequences to identify an …
Barcoded viral tracing of single-cell interactions in central nervous system inflammation
…, P Lotfy, A Tejeda-Velarde, P Hewson… - Science, 2021 - science.org
INTRODUCTION Glial cells of the central nervous system (CNS), including astrocytes and
microglia, play critical roles in development, tissue repair, and homeostasis. However, …
microglia, play critical roles in development, tissue repair, and homeostasis. However, …
[HTML][HTML] Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome
Large serine recombinases (LSRs) are DNA integrases that facilitate the site-specific integration
of mobile genetic elements into bacterial genomes. Only a few LSRs, such as Bxb1 and …
of mobile genetic elements into bacterial genomes. Only a few LSRs, such as Bxb1 and …
[PDF][PDF] Impaired local intrinsic immunity to SARS-CoV-2 infection in severe COVID-19
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both represent a term resent a l., 2019) 2019). Togeth . Toge epithelial cell co thelial ce pes in …
both represent a term resent a l., 2019) 2019). Togeth . Toge epithelial cell co thelial ce pes in …
[PDF][PDF] Structural basis for the RNA-guided ribonuclease activity of CRISPR-Cas13d
CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic
adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-…
adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-…
Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA targeting
… significantly different at p < 0.0001 in HeLa and p < 0.001 in U2OS based on Welch’st test. …
Red dots denote significantly affected transcripts with adjusted p value < 0.1 and absolute …
Red dots denote significantly affected transcripts with adjusted p value < 0.1 and absolute …
Large-scale discovery of recombinases for integrating DNA into the human genome
Recent microbial genome sequencing efforts have revealed a vast reservoir of mobile genetic
elements containing integrases that could be useful genome engineering tools. Large …
elements containing integrases that could be useful genome engineering tools. Large …
[PDF][PDF] Deep learning of Cas13 guide activity from high-throughput gene essentiality screening
Transcriptome engineering requires flexible RNA-targeting technologies that can perturb
mammalian transcripts in a robust and scalable manner. CRISPR systems that natively target …
mammalian transcripts in a robust and scalable manner. CRISPR systems that natively target …
[HTML][HTML] More than just content: building community in the graduate classroom
…, F Fernandez, AC Kwong, YJ Liu, P Lotfy… - Nature …, 2021 - nature.com
Community building should be integrated into the graduate classroom to foster the
development of a professional identity and build connections between students and faculty.
development of a professional identity and build connections between students and faculty.
[HTML][HTML] Severe COVID-19 is associated with fungal colonization of the nasopharynx and potent induction of IL-17 responses in the nasal epithelium
… We considered genes as differentially expressed with an FDR-adjusted p value < 0.05. …
We considered genes as differentially expressed with an FDR-adjusted p value < 0.001 and log …
We considered genes as differentially expressed with an FDR-adjusted p value < 0.001 and log …