Pseudomonas aeruginosa thrives in many aqueous environments and is an opportunistic
pathogen that can cause both acute and chronic infections. Environmental conditions and host …

Bacterial biofilms present a significant medical challenge because they are recalcitrant to
current therapeutic regimes. A key component of biofilm formation in the opportunistic human …

While complex intra- and interspecies microbial community dynamics are apparent during
chronic infections and likely alter patient health outcomes, our understanding of these …

Type IVa pili are protein filaments essential for virulence in many bacterial pathogens; they
extend and retract from the surface of bacterial cells to pull the bacteria forward. The motor …

The arms race between bacteria and phages has led to the evolution of diverse anti-phage
defenses, several of which are controlled by quorum-sensing pathways. In this work, we …

The exopolysaccharide Psl contributes to biofilm structure and antibiotic tolerance and may
play a role in the failure to eradicate Pseudomonas aeruginosa from cystic fibrosis (CF) …

Most bacteriophages possess long tails, which serve as the conduit for genome delivery.
We report the solution structure of the N-terminal domain of gpV, the protein comprising the …

Biofilm formation is a complex, ordered process. In the opportunistic pathogen Pseudomonas
aeruginosa, Psl and Pel exopolysaccharides and extracellular DNA (eDNA) serve as …

Extracellular polysaccharides comprise a major component of the biofilm matrix. Many
species that are adept at biofilm formation have the capacity to produce multiple types of …

Allelic exchange is an efficient method of bacterial genome engineering. This protocol
describes the use of this technique to make gene knockouts and knock-ins, as well as single-…