RNA-sequencing (RNA-seq) has a wide variety of applications, but no single analysis
pipeline can be used in all cases. We review all of the major steps in RNA-seq data analysis, …

… It next computes a p-value for significance of enrichment according to the percentage
allocation of reads in each type of regions. Based on the empirical p-value distribution computed …

The study of biliary disease has been constrained by a lack of primary human cholangiocytes.
Here we present an efficient, serum-free protocol for directed differentiation of human …

Recent studies involving induced pluripotent stem cell (iPSC) reprogramming in mice and
monocyte-to-macrophage differentiation in humans have revealed a role for the transcription …

… nonexpressed probes using the detection P value from Illumina. Across all samples, probes
for which the intensity value was not statistically significantly different (P > 0.01) from those of …

The TGFβ pathway has essential roles in embryonic development, organ homeostasis,
tissue repair and disease 1 , 2 . These diverse effects are mediated through the intracellular …

… There were significantly more differences between day 4 to 8 than between the earlier time
points (P <2.2e-16, χ 2 test) (see Additional file 9: Table S6). A total of 1,304 DHSs (11.8% of …

… eQTL that are specific to a single stage, shared across two stages, or observed across all
stages (sharing defined as a lead eQTL variant at one stage with nominal significant effects P < …

… In this case, the proportion of unique genes did not significantly differ between the two
genotypes (P = 0.1, Chi-square test). As for SVP, FLC peaks were mainly located in promoter …

Coordination of differentiation and cell cycle progression represents an essential process
for embryonic development and adult tissue homeostasis. These mechanisms ultimately …