Circadian rhythms are essential to the temporal regulation of molecular processes in living
systems and as such to life itself. Deregulation of these rhythms leads to failures in biological …

In the past years, recombinase-based approaches for integrating transgenes into defined
chromosomal loci of mammalian cells have gained increasing attention. This method is …

Colorectal cancer (CRC) is characterized by recurrent mutations deregulating key cell
signaling cascades and providing the cancer cells with novel functional traits. Among the most …

Oncogenic mutations in KRAS or BRAF are frequent in colorectal cancer and activate the
ERK kinase. Here, we find graded ERK phosphorylation correlating with cell differentiation in …

Background Pancreatic neuroendocrine neoplasms (PanNENs) fall into two subclasses: the
well-differentiated, low- to high-grade pancreatic neuroendocrine tumors (PanNETs), and …

Objective Dysregulation of the extracellular matrix molecule biglycan (BGN) predicts poor
survival in several cancer entities. Our study investigated the prognostic impact of BGN in …

Oncoproteins such as the BRAF V600E kinase endow cancer cells with malignant properties,
but they also create unique vulnerabilities. Targeting of BRAF V600E -driven cytoplasmic …

Colon cancer cells frequently carry mutations that activate the β-catenin and mitogen-activated
protein kinase (MAPK) signaling cascades. Yet how oncogenic alterations interact to …

Recombinant mouse strains that harbor tightly controlled transgene expression proved to be
indispensible tools to elucidate gene function. Different strategies have been employed to …

Faithful expression of transgenes in cell cultures and mice is often challenged by locus
dependent epigenetic silencing. We investigated silencing of Tet-controlled expression cassettes …