The Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic
loci by means of single-guide RNAs (sgRNAs) to enable genome editing 1 , 2 , 3 , 4 , 5 , 6 , …

Recent advances in genome engineering technologies based on the CRISPR-associated
RNA-guided endonuclease Cas9 are enabling the systematic interrogation of mammalian …

Appropriate use and interpretation of serological tests for assessments of severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2) exposure, infection and potential immunity …

Functional elucidation of causal genetic variants and elements requires precise genome
editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short …

Targeted genome editing technologies have enabled a broad range of research and medical
applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted to …

Targeted nucleases are powerful tools for mediating genome alteration with high precision.
The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short …

Systematic interrogation of gene function requires the ability to perturb gene expression in a
robust and generalizable manner. Here we describe structure-guided engineering of a …

The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by
single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus pyogenes …

Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-guided genome
editing and transcription regulation in eukaryotic cells, yet their in vivo target specificity is …

The dynamic nature of gene expression enables cellular programming, homeostasis and
environmental adaptation in living systems. Dissection of causal gene functions in cellular and …