Compartmentalized biochemical activities are essential to all cellular processes, but there is
no generalizable method to visualize dynamic protein activities in living cells at a resolution …

Superresolution fluorescence microscopy overcomes the diffraction resolution barrier and
allows the molecular intricacies of life to be revealed with greatly enhanced detail. However, …

Reversible photoswitching of individual molecules has been demonstrated for a number of
mutants of the green fluorescent protein (GFP). To date, however, a limited number of …

In this Perspective we discuss recent trends in the development and applications of
fluorescent proteins. We start by providing a historical and structural perspective of their …

Optical microscopes, often referred to as ‘light microscopes’, use visible light and a system
of lenses to provide us with magnified images of small samples. Combined with highly …

Advanced fluorescence imaging, including subdiffraction microscopy, relies on fluorophores
with controllable emission properties. Chief among these fluorophores are the …

Super-resolution optical fluctuation imaging (SOFI) allows one to perform sub-diffraction
fluorescence microscopy of living cells. By analyzing the acquired image sequence with an …

We present Localizer, a freely available and open source software package that implements
the computational data processing inherent to several types of superresolution fluorescence …

For almost a century, heterogeneous catalysts have been at the heart of countless industrial
chemical processes, but their operation at the molecular level is generally much less …

Monomeric red fluorescent proteins (mRFPs) have become indispensable tools for studying
protein dynamics, interactions and functions in the cellular environment. Their emission …