Targeting genomic loci by massively parallel sequencing requires new methods to enrich
templates to be sequenced. We developed a capture method that uses biotinylated RNA 'baits' …

Learning to read and write the transcriptional regulatory code is of central importance to
progress in genetic analysis and engineering. Here we describe a massively parallel reporter …

Genome-wide chromatin annotations have permitted the mapping of putative regulatory
elements across multiple human cell types. However, their experimental dissection by directed …

Genome-wide association studies (GWAS) have successfully identified thousands of associations
between common genetic variants and human disease phenotypes, but the majority of …

Targeted RNA-Seq combines next-generation sequencing with capture of sequences from
a relevant subset of a transcriptome. When testing by capturing sequences from a tumor …

Deep mutational scanning has emerged as a promising tool for mapping sequence–activity
relationships in proteins, ribonucleic acid and deoxyribonucleic acid. In this approach, …

Enhancers regulate gene expression through the binding of sequence-specific transcription
factors (TFs) to cognate motifs. Various features influence TF binding and enhancer function…

Plasmodium vivax is a major public health burden, responsible for the majority of malaria
infections outside Africa. We explored the impact of demographic history and selective …

Massively parallel reporter assays (MPRAs) enable nucleotide-resolution dissection of
transcriptional regulatory regions, such as enhancers, but only few regions at a time. Here we …

Genome-wide association studies have struggled to identify functional genes and variants
underlying complex phenotypes. We recruited a multi-ethnic cohort of healthy volunteers (n = …