Structural variations are the greatest source of genetic variation, but they remain poorly
understood because of technological limitations. Single-molecule long-read sequencing has the …

Gene expression profiling by high-throughput sequencing reveals qualitative and quantitative
changes in RNA species at steady state but obscures the intracellular dynamics of RNA …

When choosing a read mapper, one faces the trade off between speed and the ability to
map reads in highly polymorphic regions. Here, we report NextGenMap, a fast and accurate …

Defining direct targets of transcription factors and regulatory pathways is key to
understanding their roles in physiology and disease. We combined SLAM-seq [thiol(SH)–linked …

Lamins are components of the peripheral nuclear lamina and interact with heterochromatic
genomic regions, termed lamina-associated domains (LADs). In contrast to lamin B1 being …

The SK-BR-3 cell line is one of the most important models for HER2+ breast cancers, which
affect one in five breast cancer patients. SK-BR-3 is known to be highly rearranged, although …

Background Methods to read out naturally occurring or experimentally introduced nucleic
acid modifications are emerging as powerful tools to study dynamic cellular processes. The …

As the Lyme disease bacterium Borrelia burgdorferi traverses its enzootic cycle, alternating
between a tick vector and a vertebrate host, the spirochete must adapt and persist in the tick …

Hfq is a global regulator of gene expression in bacteria undergoing adaptation to changing
environmental conditions. Its major function is to promote RNA-RNA interactions between …

In search for RNA signals that modulate transcription via direct interaction with RNA
polymerase (RNAP), we deep sequenced an E. coli genomic library enriched for RNAP-binding …