Double-stranded RNA (dsRNA) directs the sequence-specific degradation of mRNA through
a process known as RNA interference (RNAi). Using a recently developed Drosophila in …

A key step in RNA interference (RNAi) is assembly of the RISC, the protein-siRNA complex
that mediates target RNA cleavage. Here, we show that the two strands of an siRNA duplex …

The 21-nucleotide small temporal RNA (stRNA) let-7regulates developmental timing in
Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro …

… We thank D. Bartel and members of the Zamore laboratory for discussions and comments
on the manuscript. We also thank M. Moore for the generous gift of HeLa extracts and G. …

Since the discovery in 1993 of the first small silencing RNA, a dizzying number of small
RNA classes have been identified, including microRNAs (miRNAs), small interfering RNAs (…

Double-stranded RNA (dsRNA) directs gene-specific, post-transcriptional silencing in many
organisms, including vertebrates, and has provided a new tool for studying gene function. …

We examined the role of ATP in the RNA interference (RNAi) pathway. Our data reveal two
ATP-dependent steps and suggest that the RNAi reaction comprises at least four sequential …

In the Drosophila and mammalian RNA interference pathways, siRNAs direct the protein
Argonaute2 (Ago2) to cleave corresponding mRNA targets, silencing their expression. Ago2 is …

Small RNA guides—microRNAs, small interfering RNAs, and repeat-associated small
interfering RNAs, 21 to 30 nucleotides in length—shape diverse cellular pathways, from …

In the Drosophila germline, repeat-associated small interfering RNAs (rasiRNAs) ensure
genomic stability by silencing endogenous selfish genetic elements such as retrotransposons …