Macrophages play a central role in the balance and efficiency of the immune response and
are at the interface between innate and adaptive immunity. Their phenotype is a delicate …

… RNA from these samples was subjected to specific microRNA RT qPCR, and miR-155 levels
… C, the same RNA extracts were also used to perform standard RT qPCR, to detect PU.1 …

Transforming growth factor-beta (TGF-β) is a pleiotropic cytokine with important effects on
processes such as fibrosis, angiogenesis, and immunosupression. Using bioinformatics, we …

… Despite these two caveats and a relatively small sample size, we observe significant positive
correlation between RT-PCR and computational estimates of ΔΨ (Fig. 2A; Supplemental …

… We validated our array data by performing specific RT-qPCR assays from BECs isolated
from additional donors (13 healthy and 15 asthmatics). Figure 1B shows that we confirmed that …

… Briefly, sections were rinsed in PBS and fixed in ice-cold (−20 C) ethanol at RT for 10 min.
Tissue sections were rehydrated in a 1:1 mixture of ethanol and water for 5 min and in PBS for …

Rationale: Asthma is one of the most common chronic diseases worldwide, and individuals
with severe asthma experience recurrent exacerbations. Exacerbations are predominantly …

… We also compared different one-step RT-qPCR kits to amplify … , and three different one-step
RT-qPCR kits achieve almost … To determine the efficiency of the RT-qPCR we serially diluted …

… We then extracted RNA and performed RT-qPCR. Interleukin-13 receptor α-1 mRNA
expression was found downregulated in biopsies from inflamed tissue compared to uninflamed …

… Confirming our RT-qPCR data, analysis of quality RNA by the Bioanalyser showed an
absence of large rRNA and a peak of small RNA in the region of 25 to 100 nt, as expected (…