Carbon catabolite repression (CCR) in bacteria is generally regarded as a regulatory
mechanism to ensure sequential utilization of carbohydrates. Selection of the carbon sources is …

… The substitutions I 371 T and R 384 G result in a slight displacement of the SXN motif,
leading to a more accessible ‘open’ active site, and it has been suggested that branched …

Foil gas bearings are self-acting hydrodynamic bearings made from sheet metal foils
comprised of at least two layers. The innermost “top foil” layer traps a gas pressure film that …

Protein interactions form a network whose structure drives cellular function and whose
organization informs biological inquiry. Using high-throughput affinity-purification mass …

We purified, cloned, and expressed aggrecanase, a protease that is thought to be
responsible for the degradation of cartilage aggrecan in arthritic diseases. Aggrecanase-1 [a …

The physiology of a cell can be viewed as the product of thousands of proteins acting in
concert to shape the cellular response. Coordination is achieved in part through networks of …

Aggrecan is responsible for the mechanical properties of cartilage. One of the earliest changes
observed in arthritis is the depletion of cartilage aggrecan due to increased proteolytic …

Mutations in SCN2A, a gene encoding the voltage-gated sodium channel Na v 1.2, have
been associated with a spectrum of epilepsies and neurodevelopmental disorders. Here, we …

Thousands of interactions assemble proteins into modules that impart spatial and functional
organization to the cellular proteome. Through affinity-purification mass spectrometry, we …

A system for high-efficiency gene replacement in Staphylococcus carnosus and Staphylococcus
xylosus has been developed, that is based on temperature-sensitive Escherichia coli–…