Over the last decade, ∼20–30 nucleotide RNA molecules have emerged as critical regulators
in the expression and function of eukaryotic genomes. Two primary categories of these …

The role of small RNAs as key regulators of mRNA turnover and translation has been well
established. Recent advances indicate that the small RNAs termed microRNAs play important …

In animals, systemic control of metabolism is conducted by metabolic tissues and relies on
the regulated circulation of a plethora of molecules, such as hormones and lipoprotein …

We investigated the potential of double-stranded RNA to interfere with the function of genes
in Drosophila. Injection of dsRNA into embryos resulted in potent and specific interference of …

The RNase III enzyme Dicer processes RNA into siRNAs and miRNAs, which direct a RNA-induced
silencing complex (RISC) to cleave mRNA or block its translation (RNAi). We have …

One of the key characteristics of stem cells is their capacity to divide for long periods of time
in an environment where most of the cells are quiescent. Therefore, a critical question in …

Innate immunity against bacterial and fungal pathogens is mediated by Toll and immune
deficiency (Imd) pathways, but little is known about the antiviral response in Drosophila. Here, …

RNA-mediated interference (RNAi) is a recently discovered method to determine gene function
in a number of organisms, including plants 1, nematodes 2, Drosophila 3, 4, zebrafish 5, …

We use native gel electrophoresis to characterize complexes that mediate RNA interference
(RNAi) in Drosophila. Our data reveal three distinct complexes (R1, R2, and R3) that …

The microRNA miR-7 is perfectly conserved from annelids to humans, and yet some of the
genes that it regulates in Drosophila are not regulated in mammals. We have explored the …