Cryo-electron microscopy (cryo-EM) has emerged as a powerful structure determination
technique. Its most prolific branch is single particle analysis (SPA), a method being used in a …

… Radostin Danev is a co-inventor with no financial benefits in US patent US9129774 B2 ‘Method
of using a phase plate in a transmission electron microscope’. Wolfgang Baumeister is …

The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here
we combined recent developments in cryo–electron tomography (cryo-ET) to produce three-…

We describe a phase plate for transmission electron microscopy taking advantage of a
hitherto-unknown phenomenon, namely a beam-induced Volta potential on the surface of a …

Class B G-protein-coupled receptors are major targets for the treatment of chronic diseases,
such as osteoporosis, diabetes and obesity. Here we report the structure of a full-length …

Phase contrast transmission electron microscopy (TEM) based on thin-film phase plates has
been developed and applied to biological systems. Currently, development is focused on …

… The spatial frequency k h is related to the hole radius r h through the formula:(3) k h = r h fλ
, where f is the focal length of the objective. Effects such as charging or contamination could …

… Further comparison of our active A 1 R structure with either of the A 2A R active or … sites
between agonist-bound A 1 R and A 2A R, suggesting that the propagation and selection of …

The 26S proteasome is a key player in eukaryotic protein quality control and in the regulation
of numerous cellular processes. Here, we describe quantitative in situ structural studies of …

… We also report the structure of the A 1 R co-bound to adenosine, MIPS521 and a G i2
heterotrimer, revealing an extrahelical lipid–detergent-facing allosteric binding pocket that involves …