Trans‐acting translational regulatory RNA binding proteins

…, TS Smith, T Mulroney, RML Queiroz… - Wiley …, 2018 - Wiley Online Library
The canonical molecular machinery required for global mRNA translation and its control
has been well defined, with distinct sets of proteins involved in the processes of translation …

Comprehensive identification of RNA–protein interactions in any organism using orthogonal organic phase separation (OOPS)

RML Queiroz, T Smith, E Villanueva… - Nature …, 2019 - nature.com
Existing high-throughput methods to identify RNA-binding proteins (RBPs) are based on
capture of polyadenylated RNAs and cannot recover proteins that interact with nonadenylated …

[HTML][HTML] Organic phase separation opens up new opportunities to interrogate the RNA-binding proteome

T Smith, E Villanueva, RML Queiroz… - Current opinion in …, 2020 - Elsevier
Protein–RNA interactions regulate all aspects of RNA metabolism and are crucial to the function
of catalytic ribonucleoproteins. Until recently, the available technologies to capture RNA-…

[HTML][HTML] System-wide analysis of RNA and protein subcellular localization dynamics

…, T Smith, M Pizzinga, M Elzek, RML Queiroz… - Nature …, 2024 - nature.com
Although the subcellular dynamics of RNA and proteins are key determinants of cell homeostasis,
their characterization is still challenging. Here we present an integrative framework to …

[HTML][HTML] Changes in the oligodendrocyte progenitor cell proteome with ageing

AG de la Fuente, RML Queiroz, T Ghosh… - Molecular & Cellular …, 2020 - ASBMB
Following central nervous system (CNS) demyelination, adult oligodendrocyte progenitor
cells (OPCs) can differentiate into new myelin-forming oligodendrocytes in a regenerative …

[HTML][HTML] Analysis of Phaseolus vulgaris response to its association with Trichoderma harzianum (ALL-42) in the presence or absence of the phytopathogenic fungi …

JL Pereira, RML Queiroz, SO Charneau, CR Felix… - Plos one, 2014 - journals.plos.org
The present study was carried out to evaluate the ability of Trichoderma harzianum (ALL 42-isolated
from Brazilian Cerrado soil) to promote common bean growth and to modulate its …

[HTML][HTML] Deconjugated bile salts produced by extracellular bile-salt hydrolase-like activities from the probiotic Lactobacillus johnsonii La1 inhibit Giardia duodenalis in …

…, C Deregnaucourt, S Chaouch, RML Queiroz… - Frontiers in …, 2016 - frontiersin.org
Giardiasis, currently considered a neglected disease, is caused by the intestinal protozoan
parasite Giardia duodenalis and is widely spread in human as well as domestic and wild …

[HTML][HTML] Two β-xylanases from Aspergillus terreus: characterization and influence of phenolic compounds on xylanase activity

…, LP Silva, CAO Ricart, PA Martins, RML Queiroz… - Fungal genetics and …, 2013 - Elsevier
Sugarcane bagasse was used as an inexpensive alternative carbon source for production
of β-xylanases from Aspergillus terreus. The induction profile showed that the xylanase …

[HTML][HTML] Quantitative proteomic and phosphoproteomic analysis of Trypanosoma cruzi amastigogenesis

RML Queiroz, S Charneau, SC Mandacaru… - Molecular & Cellular …, 2014 - ASBMB
Chagas disease is a tropical neglected disease endemic in Latin America caused by the
protozoan Trypanosoma cruzi. The parasite has four major life stages: epimastigote, metacyclic …

Efficient recovery of the RNA-bound proteome and protein-bound transcriptome using phase separation (OOPS)

E Villanueva, T Smith, RML Queiroz, M Monti… - Nature protocols, 2020 - nature.com
RNA−protein interactions play a pivotal role in cell homeostasis and disease, but current
approaches to study them require a considerable amount of starting material, favor the recovery …