The potential usefulness of human embryonic stem cells for therapy derives from their ability
to form any cell in the body. This potential has been used to justify intensive research …

Aim: Determination of the phenotype of adult human atrial and ventricular myocytes based
on gene expression and morphology. Methods: Atrial and ventricular cardiomyocytes were …

Rationale: There are several methods to measure cardiomyocyte and muscle contraction,
but these require customized hardware, expensive apparatus, and advanced informatics or …

… (R min ) after addition of 5 μg/mL ionomycin and 4 mmol/L EGTA (pH 8) to the cells and the
maximal ratio (R … The calcium concentration was calculated as follows: (R−R min )/(R max −R)…

Hypertrophic growth is an adaptive response of the heart to diverse pathological stimuli and
is characterized by cardiomyocyte enlargement, sarcomere assembly, and activation of a …

Although it is generally accepted that cellular differentiation requires changes to transcriptional
networks, dynamic regulation of promoters and enhancers at specific sets of genes has …

Defined growth conditions are essential for many applications of human embryonic stem
cells (hESC). Most defined media are presently used in combination with Matrigel, a partially …

NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5
locus of human embryonic stem cells (hESCs); NKX2-5 eGFP/w hESCs facilitate …

Recent withdrawals of prescription drugs from clinical use because of unexpected side
effects on the heart have highlighted the need for more reliable cardiac safety pharmacology …

Human embryonic stem cells (hESCs) can differentiate into cardiomyocytes, but the efficiency
of this process is low. We routinely induce cardiomyocyte differentiation of the HES‐2 cell …