Generation of knock-in primary human T cells using Cas9 ribonucleoproteins
…, M Subramaniam, RE Gate… - Proceedings of the …, 2015 - National Acad Sciences
T-cell genome engineering holds great promise for cell-based therapies for cancer, HIV,
primary immune deficiencies, and autoimmune diseases, but genetic manipulation of human T …
primary immune deficiencies, and autoimmune diseases, but genetic manipulation of human T …
Genetic determinants of co-accessible chromatin regions in activated T cells across humans
Over 90% of genetic variants associated with complex human traits map to non-coding regions,
but little is understood about how they modulate gene regulation in health and disease. …
but little is understood about how they modulate gene regulation in health and disease. …
Multiplexed droplet single-cell RNA-sequencing using natural genetic variation
…, E Wan, S Wong, L Byrnes, CM Lanata, RE Gate… - Nature …, 2018 - nature.com
Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel
profiling of transcriptomes. However, assessing differential expression across multiple …
profiling of transcriptomes. However, assessing differential expression across multiple …
[HTML][HTML] CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells
Immunotherapies with chimeric antigen receptor (CAR) T cells and checkpoint inhibitors (including
antibodies that antagonize programmed cell death protein 1 [PD-1]) have both …
antibodies that antagonize programmed cell death protein 1 [PD-1]) have both …
Discovery of stimulation-responsive immune enhancers with CRISPR activation
The majority of genetic variants associated with common human diseases map to enhancers,
non-coding elements that shape cell-type-specific transcriptional programs and responses …
non-coding elements that shape cell-type-specific transcriptional programs and responses …
A tissue checkpoint regulates type 2 immunity
…, AB Molofsky, HE Liang, JL Pollack, RE Gate… - Nature …, 2016 - nature.com
Group 2 innate lymphoid cells (ILC2s) and CD4 + type 2 helper T cells (T H 2 cells) are defined
by their similar effector cytokines, which together mediate the features of allergic immunity…
by their similar effector cytokines, which together mediate the features of allergic immunity…
Functional CRISPR dissection of gene networks controlling human regulatory T cell identity
Human regulatory T (T reg ) cells are essential for immune homeostasis. The transcription
factor FOXP3 maintains T reg cell identity, yet the complete set of key transcription factors that …
factor FOXP3 maintains T reg cell identity, yet the complete set of key transcription factors that …
[PDF][PDF] A calmodulin C-lobe Ca2+-dependent switch governs Kv7 channel function
…, F Abderemane-Ali, GL Hura, ND Rossen, RE Gate… - Neuron, 2018 - cell.com
Kv7 (KCNQ) voltage-gated potassium channels control excitability in the brain, heart, and
ear. Calmodulin (CaM) is crucial for Kv7 function, but how this calcium sensor affects activity …
ear. Calmodulin (CaM) is crucial for Kv7 function, but how this calcium sensor affects activity …
Determination of ubiquitin fitness landscapes under different chemical stresses in a classroom setting
…, SD Axen, E Caceres, W Chen, A Cuesta, RE Gate… - Elife, 2016 - elifesciences.org
10.7554/eLife.15802.001 Ubiquitin is essential for eukaryotic life and varies in only 3 amino
acid positions between yeast and humans. However, recent deep sequencing studies …
acid positions between yeast and humans. However, recent deep sequencing studies …
Genetic analysis of isoform usage in the human anti-viral response reveals influenza-specific regulation of ERAP2 transcripts under balancing selection
While genetic variants are known to be associated with overall gene abundance in stimulated
immune cells, less is known about their effects on alternative isoform usage. By analyzing …
immune cells, less is known about their effects on alternative isoform usage. By analyzing …