Strategies for stabilization of enzymes in organic solvents
…, T Koudelakova, Z Prokop, R Chaloupkova… - Acs …, 2013 - ACS Publications
One of the major barriers to the use of enzymes in industrial biotechnology is their insufficient
stability under processing conditions. The use of organic solvent systems instead of …
stability under processing conditions. The use of organic solvent systems instead of …
Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate
M Pavlova, M Klvana, Z Prokop, R Chaloupkova… - Nature chemical …, 2009 - nature.com
Engineering enzymes to degrade anthropogenic compounds efficiently is challenging. We
obtained Rhodococcus rhodochrous haloalkane dehalogenase mutants with up to 32-fold …
obtained Rhodococcus rhodochrous haloalkane dehalogenase mutants with up to 32-fold …
[HTML][HTML] Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway
…, R Fedr, K Soucek, M Sedlackova, R Chaloupkova… - Microbial cell …, 2015 - Springer
Background Heterologous expression systems based on promoters inducible with isopropyl-β-D-1-thiogalactopyranoside
(IPTG), eg, Escherichia coli BL21(DE3) and cognate LacI Q /P …
(IPTG), eg, Escherichia coli BL21(DE3) and cognate LacI Q /P …
Haloalkane dehalogenases: biotechnological applications
Haloalkane dehalogenases (EC 3.8.1.5, HLDs) are α/β‐hydrolases which act to cleave
carbon‐halogen bonds. Due to their unique catalytic mechanism, broad substrate specificity and …
carbon‐halogen bonds. Due to their unique catalytic mechanism, broad substrate specificity and …
Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel
T Koudelakova, R Chaloupkova… - Angewandte …, 2013 - Wiley Online Library
Biocatalysts produced by mesophilic organisms exhibit high selectivity and operability under
environmentally friendly conditions.[1] However, their practical applications can be limited …
environmentally friendly conditions.[1] However, their practical applications can be limited …
[HTML][HTML] FireProt: energy-and evolution-based computational design of thermostable multiple-point mutants
There is great interest in increasing proteins’ stability to enhance their utility as biocatalysts,
therapeutics, diagnostics and nanomaterials. Directed evolution is a powerful, but …
therapeutics, diagnostics and nanomaterials. Directed evolution is a powerful, but …
Enantioselectivity of haloalkane dehalogenases and its modulation by surface loop engineering
…, J Brezovsky, T Mozga, R Chaloupkova… - Angewandte Chemie …, 2010 - Wiley Online Library
Enzymes are widely used for the synthesis of pharmaceuticals, agrochemicals, and food
additives because they can catalyze enantioselective transformations.[1] Understanding the …
additives because they can catalyze enantioselective transformations.[1] Understanding the …
[HTML][HTML] Catalytic mechanism of the haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26
Z Prokop, M Monincová, R Chaloupková… - Journal of Biological …, 2003 - ASBMB
Haloalkane dehalogenases are bacterial enzymes capable of carbon-halogen bond cleavage
in halogenated compounds. To obtain insights into the mechanism of the haloalkane …
in halogenated compounds. To obtain insights into the mechanism of the haloalkane …
Pathways and mechanisms for product release in the engineered haloalkane dehalogenases explored using classical and random acceleration molecular dynamics …
…, M Pavlova, T Koudelakova, R Chaloupkova… - Journal of molecular …, 2009 - Elsevier
Eight mutants of the DhaA haloalkane dehalogenase carrying mutations at the residues lining
two tunnels, previously observed by protein X-ray crystallography, were constructed and …
two tunnels, previously observed by protein X-ray crystallography, were constructed and …
Engineering a de novo transport tunnel
…, IK Smatanova, Z Prokop, R Chaloupkova… - ACS …, 2016 - ACS Publications
Transport of ligands between buried active sites and bulk solvent is a key step in the catalytic
cycle of many enzymes. The absence of evolutionary optimized transport tunnels is an …
cycle of many enzymes. The absence of evolutionary optimized transport tunnels is an …