We describe a phase plate for transmission electron microscopy taking advantage of a
hitherto-unknown phenomenon, namely a beam-induced Volta potential on the surface of a …

The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here
we combined recent developments in cryo–electron tomography (cryo-ET) to produce three-…

Class B G-protein-coupled receptors are major targets for the treatment of chronic diseases,
such as osteoporosis, diabetes and obesity. Here we report the structure of a full-length …

The possibility of implementing a Zernike phase plate in a transmission electron microscope
is investigated both theoretically and experimentally. The phase-retarding plate in the form …

The 26S proteasome is a key player in eukaryotic protein quality control and in the regulation
of numerous cellular processes. Here, we describe quantitative in situ structural studies of …

The class A adenosine A 1 receptor (A 1 R) is a G-protein-coupled receptor that preferentially
couples to inhibitory G i/o heterotrimeric G proteins, has been implicated in numerous …

The class B glucagon-like peptide-1 (GLP-1) G protein-coupled receptor is a major target for
the treatment of type 2 diabetes and obesity 1 . Endogenous and mimetic GLP-1 peptides …

With the advent of direct electron detectors, the perspectives of cryo-electron microscopy (cryo-EM)
have changed in a profound way. These cameras are superior to previous detectors …

In situ cryo electron tomography of cryo focused ion beam milled samples has emerged in
recent years as a powerful technique for structural studies of macromolecular complexes in …

The adenosine A 1 receptor (A 1 R) is a promising therapeutic target for non-opioid analgesic
agents to treat neuropathic pain 1 , 2 . However, development of analgesic orthosteric A 1 …