Massively parallel sequencing of cDNA has enabled deep and efficient probing of transcriptomes.
Current approaches for transcript reconstruction from such data often rely on aligning …

Massively-parallel cDNA sequencing has opened the way to deep and efficient probing of
transcriptomes. Current approaches for transcript reconstruction from such data often rely on …

Recent molecular studies have shown that, even when derived from a seemingly homogenous
population, individual cells can exhibit substantial differences in gene expression, protein …

Intestinal epithelial cells absorb nutrients, respond to microbes, function as a barrier and
help to coordinate immune responses. Here we report profiling of 53,193 individual epithelial …

Genetic screens help infer gene function in mammalian cells, but it has remained difficult to
assay complex phenotypes—such as transcriptional profiles—at scale. Here, we develop …

High-throughput single-cell transcriptomics offers an unbiased approach for understanding
the extent, basis and function of gene expression variation between seemingly identical cells…

Background & Aims: Hypergastrinemia occurs frequently in association with acid suppression
and Helicobacter infection, but its role in the progression to gastric atrophy and gastric …

Cellular RNA levels are determined by the interplay of RNA production, processing and
degradation. However, because most studies of RNA regulation do not distinguish the separate …

Finding the components of cellular circuits and determining their functions systematically
remains a major challenge in mammalian cells. Here, we introduced genome-wide pooled …

INTRODUCTION Mammalian gene expression is tightly controlled through the interplay
between the RNA and protein life cycles. Although studies of individual genes have shown that …