User profiles for Richie E. Kohman
Richie KohmanWyss Center for Bio and Neuroengineering Verified email at mit.edu Cited by 1341 |
Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems
INTRODUCTION Cells and tissues are made up of diverse molecular building blocks,
organized with nanoscale precision over extended length scales. Newly developed techniques …
organized with nanoscale precision over extended length scales. Newly developed techniques …
A comprehensive library of human transcription factors for cell fate engineering
Human pluripotent stem cells (hPSCs) offer an unprecedented opportunity to model diverse
cell types and tissues. To enable systematic exploration of the programming landscape …
cell types and tissues. To enable systematic exploration of the programming landscape …
Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression
An in vitro model of human ovarian follicles would greatly benefit the study of female
reproduction. Ovarian development requires the combination of germ cells and several types of …
reproduction. Ovarian development requires the combination of germ cells and several types of …
Light-triggered release of bioactive molecules from DNA nanostructures
Recent innovations in DNA nanofabrication allow the creation of intricately shaped
nanostructures ideally suited for many biological applications. To advance the use of DNA …
nanostructures ideally suited for many biological applications. To advance the use of DNA …
Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses
We present barcoded oligonucleotides ligated on RNA amplified for multiplexed and
parallel insitu analyses (BOLORAMIS), a reverse transcription-free method for spatially-resolved, …
parallel insitu analyses (BOLORAMIS), a reverse transcription-free method for spatially-resolved, …
Recovery of walking after paralysis by regenerating characterized neurons to their natural target region
Axon regeneration can be induced across anatomically complete spinal cord injury (SCI),
but robust functional restoration has been elusive. Whether restoring neurological functions …
but robust functional restoration has been elusive. Whether restoring neurological functions …
[HTML][HTML] Photon-directed multiplexed enzymatic DNA synthesis for molecular digital data storage
…, S Punthambaker, H Chun, RE Kohman… - Nature …, 2020 - nature.com
New storage technologies are needed to keep up with the global demands of data
generation. DNA is an ideal storage medium due to its stability, information density and ease-of-…
generation. DNA is an ideal storage medium due to its stability, information density and ease-of-…
Leukocyte-mimicking stem cell delivery via in situ coating of cells with a bioactive hyperbranched polyglycerol
Since stem cells emerged as a new generation of medicine, there are increasing efforts to
deliver stem cells to a target tissue via intravascular injection. However, the therapeutic stem …
deliver stem cells to a target tissue via intravascular injection. However, the therapeutic stem …
Light sensitization of DNA nanostructures via incorporation of photo-cleavable spacers
Using light irradiation as a trigger, large-scale structural reconfiguration of DNA nanostructures
is demonstrated. We incorporated photo-cleavable spacers at strategic locations within …
is demonstrated. We incorporated photo-cleavable spacers at strategic locations within …
How collaboration between bioethicists and neuroscientists can advance research
By integrating ongoing bioethical collaboration, neuroscientists can create a positive effect
on their research and the knowledge it produces. To this end, we offer our experiences with …
on their research and the knowledge it produces. To this end, we offer our experiences with …