The ability to selectively modify proteins at two or more defined locations opens new avenues
for manipulating, engineering, and studying living systems. As a chemical biology tool for …

The ability to site-specifically modify proteins at multiple sites in vivo will enable the study of
protein function in its native environment with unprecedented levels of detail. Here, we …

During integration into materials, the inactivation of enzymes as a result of their interaction
with nanometer size denaturing “hotspots” on surfaces represents a critical challenge. This …

Biomaterials based on immobilized proteins are key elements of many biomedical and
industrial technologies. However, applications are limited by an inability to precisely construct …

Assembling nanobodies (Nbs) into polyvalent multimers is a powerful strategy for improving
the effectiveness of Nb-based therapeutics and biotechnological tools. However, generally …

Genetic code expansion (GCE) technologies incorporate non-canonical amino acids (ncAAs)
into proteins at amber stop codons. To avoid unwanted truncated protein and improve …

The development of bioorthogonal fluorogenic probes constitutes a vital force to advance life
sciences. Tetrazine‐encoded green fluorescent proteins (GFPs) show high bioorthogonal …

Quantitative labeling of biomolecules is necessary to advance areas of antibody–drug
conjugation, super-resolution microscopy imaging of molecules in live cells, and determination of …

The availability of an expanded genetic code opens exciting new opportunities in enzyme
design and engineering. In this regard histidine analogues have proven particularly versatile, …

Edward Lemke and his team have recently reported the creation of a membraneless organelle
for site-specific protein engineering, achieving a functioning level of spatial orthogonality …