Genome-resolved meta-omics ties microbial dynamics to process performance in biotechnology for thiocyanate degradation

RS Kantor, RJ Huddy, R Iyer, BC Thomas… - … science & technology, 2017 - ACS Publications
Remediation of industrial wastewater is important for preventing environmental contamination
and enabling water reuse. Biological treatment for one industrial contaminant, thiocyanate …

Biological pretreatment of carbonaceous matter in double refractory gold ores: A review and some future considerations

KT Konadu, DM Mendoza, RJ Huddy, STL Harrison… - Hydrometallurgy, 2020 - Elsevier
The pretreatment of carbonaceous material in double refractory gold ores (DRGO) is
necessary to decrease preg-robbing of gold and maximize gold recovery. DRGO contains of …

[HTML][HTML] Vitamin interdependencies predicted by metagenomics-informed network analyses and validated in microbial community microcosms

T Hessler, RJ Huddy, R Sachdeva, S Lei… - Nature …, 2023 - nature.com
Metagenomic or metabarcoding data are often used to predict microbial interactions in complex
communities, but these predictions are rarely explored experimentally. Here, we use an …

Sequential pretreatment of double refractory gold ore (DRGO) with a thermophilic iron oxidizing archeaon and fungal crude enzymes

KT Konadu, RJ Huddy, STL Harrison… - Minerals …, 2019 - Elsevier
Double refractory gold ore was sequentially pretreated to oxidize sulfides by thermophilic
archaeon Acidianus brierleyi and then to decompose carbonaceous matters using the cell-free …

Characterisation of the complex microbial community associated with the ASTER™ thiocyanate biodegradation system

RJ Huddy, AW Van Zyl, RP Van Hille, STL Harrison - Minerals Engineering, 2015 - Elsevier
The ASTER™ process is used to bioremediate cyanide- (CN − ) and thiocyanate- (SCN − )
containing waste water. This aerobic process is able to reduce the CN − and SCN − …

Harnessing Fermentation May Enhance the Performance of Biological Sulfate-Reducing Bioreactors

…, STL Harrison, JF Banfield, RJ Huddy - … Science & Technology, 2024 - ACS Publications
Biological sulfate reduction (BSR) represents a promising strategy for bioremediation of
sulfate-rich waste streams, yet the impact of metabolic interactions on performance is largely …

[HTML][HTML] Stratification of microbial communities throughout a biological sulphate reducing up-flow anaerobic packed bed reactor, revealed through 16S metagenomics

T Hessler, STL Harrison, RJ Huddy - Research in microbiology, 2018 - Elsevier
Biological sulphate reduction (BSR) is a promising low-cost treatment of acid rock drainage
effluents. In this paper, the system performance and microbial ecology of a lactate …

An unusual feruloyl esterase belonging to family VIII esterases and displaying a broad substrate range

…, DLR Mutepfa, A Casanueva, RJ Huddy… - Journal of Molecular …, 2015 - Elsevier
A thermophilic compost metagenomic library constructed in Escherichia coli was functionally
screened for novel esterases. Of the 110,592 fosmid clones screened, 25 clones …

Linking Microbial Community Dynamics in BIOX® Leaching Tanks to Process Conditions: Integrating Lab and Commercial Experience

M Smart, RJ Huddy, CJ Edward, C Fourie… - Solid State …, 2017 - Trans Tech Publ
In the commercial BIOX ® process, an acidophilic mixed bacterial and archaeal community
dominated by iron and sulphur oxidising microorganisms is used to facilitate the recovery of …

[HTML][HTML] Detection and localisation of the abalone probiotic Vibrio midae SY9 and its extracellular protease, VmproA, within the digestive tract of the South African …

RJ Huddy, VE Coyne - PloS one, 2014 - journals.plos.org
Probiotics have been widely reported to increase the growth rate of commercially important
fish and shellfish by enhancing the digestion of ingested feed through the production of …