Bacterial polyhydroxyalkanoates (PHA) are polyesters accumulated as carbon and energy
storage materials under limited growth conditions in the presence of excess carbon sources. …

During cell division, kinetochore microtubules (KMTs) provide a physical linkage between
the chromosomes and the rest of the spindle. KMTs in mammalian cells are organized into …

Spindle microtubules, whose dynamics vary over time and at different locations, cooperatively
drive chromosome segregation. Measurements of microtubule dynamics and spindle …

During eukaryotic cell division, chromosomes are linked to microtubules (MTs) in the spindle
by a macromolecular complex called the kinetochore. The bound kinetochore microtubules (…

Chromosome segregation during male meiosis is tailored to rapidly generate multitudes of
sperm. Little is known about mechanisms that efficiently partition chromosomes to produce …

We present a software‐assisted workflow for the alignment and matching of filamentous
structures across a three‐dimensional (3D) stack of serial images. This is achieved by …

The mitotic spindle is a complex three-dimensional (3D) apparatus that functions to ensure
the faithful segregation of chromosomes during cell division. Our current understanding of …

Faithful chromosome segregation requires the assembly of a bipolar spindle, consisting of
two antiparallel microtubule (MT) arrays having most of their minus ends focused at the …

We introduce a new workflow that allows screening and selection of staged mammalian
cells in mitosis prior to subsequent electron microscopy. We mainly describe four improved …

3D segmentation of cytoskeletal filaments and organelles is crucial for studying these
structures in cellular (cryo-) electron microscopy (EM) and tomography (ET). Manual annotation …