A genome-scale genetic interaction map was constructed by examining 5.4 million gene-gene
pairs for synthetic genetic interactions, generating quantitative genetic interaction profiles …

Genetics aims to understand the relation between genotype and phenotype. However,
because complete deletion of most yeast genes (∼80%) has no obvious phenotypic …

Sometimes mutations in two genes produce a phenotype that is surprising in light of each
mutation's individual effects. This phenomenon, which defines genetic interaction, can reveal …

Genome-wide characterization of the in vivo cellular response to perturbation is fundamental
to understanding how cells survive stress. Identifying the proteins and pathways perturbed …

Next-generation sequencing has proven an extremely effective technology for molecular
counting applications where the number of sequence reads provides a digital readout for RNA-…

Systematic genetic interaction studies have illuminated many cellular processes. Here we
quantitatively examine genetic interactions among 26 Saccharomyces cerevisiae genes …

Background Genome-scale CRISPR interference (CRISPRi) has been used in human cell
lines; however, the features of effective guide RNAs (gRNAs) in different organisms have not …

For decades, fungi have been a source of US Food and Drug Administration–approved natural
products such as penicillin, cyclosporine, and the statins. Recent breakthroughs in DNA …

… In the version of this article initially published, there was a space missing in the author name
Robert P St Onge. The error has been corrected in the HTML and PDF versions of the article…

The Saccharomyces cerevisiae sequencing project (the first eukaryotic genome decoded)
was completed in 1995 and, subsequently, the first version of the yeast knockout collection …